免疫标记技术在死后经过时间推断中的研究进展

Research progress of using immunolabeling techniques to estimate of postmortem interval

死后经过时间的准确推断是法医病理学和刑事侦查领域的一项困难且极为关键的任务。近年来,物理、化学、微生物学、免疫学、昆虫学、分子生物学等学科方兴未艾,特殊染色、光谱、质谱、色谱、影像学等技术不断发展,用于死后经过时间推断的方法日益增多,其中免疫标记技术发挥了重要作用。本文系统地综述了国内外利用免疫标记技术推断死后经过时间的相关研究,包括免疫组化技术、免疫印迹技术、免疫荧光技术、放射免疫技术等,总结并分析这些技术在死后经过时间推断中的研究进展,以期为开拓法医病理学死后经过时间推断的研究思路,并能更好地将之应用于法医实践中提供参考。

The accurate deduction of postmortem interval is a difficult and extremely critical task in the field of forensic pathology and criminal investigation.In recent years,physics,chemistry,microbiology,immunology,entomology,molecular biology and other disciplines have been on the rise,and special staining,spectroscopy,mass spectrometry,chromatography,radiographic and other techniques have been developed,and methods for postmortem interval inference are increasing,among which immunolabelling techniques have played an important role.In this paper,we systematically reviewed the domestic and foreign relevant studies on the postmortem interval inference using of immunolabelling techniques,including immunohistochemistry,immunoblotting,immunofluorescence,radioimmunoassay,etc.We summarized and analyzed the research progress on these techniques in postmortem interval inference,with the aim of exploring the ideas for the study of postmortem interval inference in forensic pathology,and provide reference for better applying them in forensic practice.