硫磺菌多糖的超声辅助复合酶法提取工艺及其抗氧化活性研究

Ultrasonic-Assisted Composite Enzyme Extraction and Antioxidant Activity of Laetiporus sulphureus Polysaccharide

为提高硫磺菌多糖的得率,分析硫磺菌多糖的结构及其抗氧化活性,文章采用超声波辅助复合酶法提取硫磺菌多糖,利用单因素及响应面实验对超声时间、酶解时间和料液比进行优化,应用高效液相色谱、傅里叶红外光谱、显微拉曼光谱等仪器分析硫磺菌多糖的结构特征,并通过测定铁离子还原能力和2,2-联氨-双-3-乙基苯并噻唑啉-6-磺酸(2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基、1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picrylhydrazyl, DPPH)自由基、羟自由基的清除能力评价其抗氧化活性。结果表明:硫磺菌多糖的最佳提取工艺为超声时间30.719 min、酶解时间79.017 min、料液比1:30.634,在此条件下,多糖的得率为(4.36±0.036)%。硫磺菌多糖由甘露糖、葡萄糖、半乳糖和岩藻糖组成,摩尔比例为22.03:42.26:28.25:7.46,为α-吡喃型多糖,分子质量分布不均一。硫磺菌多糖具有良好的体外抗氧化作用。该研究结果为硫磺菌多糖作为潜在天然抗氧化剂提供了数据支持。

In order to improve the yield of Laetiporus sulphureus polysaccharide(LSP)and analyze the structure and antioxidant activity of LSP,LSP was extracted by ultrasound-assisted complex enzymatic method.The extraction conditions were optimized by single factor and response surface method.High performance liquid chromatography(HPLC),Fourier infrared spectroscopy(FT-IR)and Raman spectroscopy(Ram)were used to analyze the structural characteristics and the antioxidant activity was evaluated by measuring the iron ion reducing ability and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS)radical cation,1,1-diphenyl-2-picrylhydrazyl(DPPH)radical,hydroxyl free radical scavenging ability.The extraction process was optimized as ultrasonic time 30.719 min,enzymatic time 79.017min,solid-liquid ratio 1:30.634.Under these conditions,the LSP yield was(4.36±0.036)%.LSP is anα-pyranose polysaccharide composed of mannose,glucose,galactose and fucose,and the molar ratio was 22.03:42.26:28.25:7.46.The molecular weight distribution was inhomogeneity.It exhibited antioxidant effect in vitro.The study provided data support for the use of Laetiporus sulphureus polysaccharide as potential natural antioxidants.