芸豆GH3基因家族鉴定及进化与表达分析

Identification and evolutional and expression analysis of the GH3 gene family in kidney bean

【目的】探究芸豆中PvGH3家族成员的特征及在非生物胁迫响应中的作用,为深入研究其基因功能提供参考依据,也为芸豆抗逆新品种的选育提供新的基因资源。【方法】通过生物信息学的方法对芸豆GH3基因家族进行全基因组鉴定,用荧光定量PCR(qRT-PCR)检测PvGH3基因在不同逆境胁迫下的表达。【结果】在芸豆中鉴定出19个PvGH3基因,其中17个基因不均匀分布于8条染色体上,其余2个基因定位于scafford上无法精确定位;可分为3个亚族,各亚族间的成员有相似的基因结构和保守基序;各成员间存在3个旁系同源基因对,与拟南芥GH3间有15个直系同源基因对;含有光、激素、胁迫及生长发育相关顺式元件;19个PvGH3在响应干旱(6%PEG)、盐(100 mmol/L NaCl溶液)和低温(4℃)逆境胁迫应答方面均上调表达。【结论】在芸豆中鉴定出19个PvGH3基因,通过qRT-PCR证明这19个PvGH3基因能不同程度地响应非生物胁迫,可为PvGH3的抗逆功能分析提供理论依据。

[Objective]This study aims to explore the characteristics of PvGH3 family members in kidney bean and their roles in abiotic stress,to provide reference for further study of gene function,as well as provides new gene resources for breeding stress tolerant varieties of kidney bean.[Methods]Genome-wide identification of the GH3 gene family in kidney bean was carried out by a bioinformatics approach,and qRT-PCR was used to detect the expression of the PvGH3 gene under abiotic stresses.[Results]19 PvGH3 genes were identified in kidney bean,of which 17 genes were unevenly distributed on eight chromosomes,and the remaining two genes were localized on scafford and could not be precisely located.These genes could be classified into three subfamilies,with members of each subfamily sharing similar gene structure and conserved motif.There were three paralogous pairs among the members,and 15 orthologous pairs with Arabidopsis.The 19 PvGH3 were up-regulated in response to drought(6%PEG),salt(100 mmol/L NaCl),and low temperature(4℃).[Conclusion]19 PvGH3 genes were identified in kidney bean.qRT-PCR assays showed that these PvGH3 genes respond to abiotic stresses to different degrees,which provided basis for the analysis of stress tolerance of PvGH3.