基于RT-PCR方法对河北省桃病毒和类病毒种类的调查鉴定

Investigation and identification of peach virus and virus-like species in Hebei Province based on RT-PCR

病毒是造成桃果实产量和品质下降的原因之一,RT-PCR是检测病毒的常用分子生物学方法。利用RT-PCR方法对河北省113份桃样品进行了13种病毒的检测,结果表明,检测到的10种病毒包括ACLSV、PBNSPaV、HSVd、NSPaV、PNRSV、PaLV、PLMVD、APCLSV、PeVD和PPV,其中ACLSV检测率最高,其次是PBNSPaV和HSVd 2种病毒。病毒具有复合侵染的现象,三重侵染和四重侵染的检测率最高,没有检测到不被病毒侵染和单一侵染的样品。研究结果为河北省桃病毒脱除、无病毒苗木繁育奠定了理论基础。

Viruses are one of the causes of peach fruit yield and quality decline,and RT-PCR is a commonly used molecular biology method to detect viruses.In this study,13 viruses were detected in 113 peach samples from Hebei Province using RT-PCR,and the results showed that 10 viruses including ACLSV,PBNSPaV,HSVd,NSPaV,PNRSV,PaLV,PLMVD,APCLSV,PeVD,and PPV were detected,among which the highest detection rate was found for ACLSV,and the highest detection rate was for PBNSPaV and HSVd the detection rate of both viruses.The viruses had the phenomenon of compound infestation,with the highest detection rates for triple and quadruple infestation,and no samples that were not infested with viruses and single infestation were detected.This study lays a theoretical foundation for peach virus removal and virus-free seedling breeding in Hebei Province.