基于表面增强拉曼光谱法的5-羟基色氨酸快速检测方法的研究

Research on rapid detection method of 5-hydroxytryptophan based on surface-enhanced Raman spectroscopy

建立了一种基于表面增强拉曼光谱法快速测定食品中5-羟基色氨酸(5-hydroxytryptophan,5-HTP)的方法。本方法采用6次平行实验,样品使用乙腈提取,1 M硝酸反萃后与增强试剂混合上机检测,可以观测到良好的增强拉曼信号,线性范围在0~20 mg/kg,检出限为1 mg/kg。使用该方法检测市售的样品,并结合平行实验验证,检测结果和HPLC方法有较高的一致性。该方法具有操作简单、快速、高效的特点。

In this paper,a method for the rapid determination of 5-hydroxytryptophan based on surface-enhanced Raman spectroscopy is established,which has the characteristics of simplicity,fastness and efficiency.This method employs six parallel experiments,and the samples are extracted with acetonitrile,back-extracted with 1 M nitric acid and mixed with the enhancement reagent for on-line detection.A good enhanced Raman signal is observed.The linear range is 0~20 mg/kg,and the detection limit is 1 mg/kg.The above method is used to detect commercially available samples,and combined with parallel experimental verification,the detection results are highly consistent with the HPLC method.