姜黄素/替莫唑胺脂质纳米粒的制备工艺研究

Preparation of Curcumin/Temozolomide Lipid Nanoparticles

目的:制备共载姜黄素(Cur)/替莫唑胺(TMZ)的脂质纳米粒(lipid nanoparticles,Cur/TMZ-LN)。方法:用薄膜分散法、反复冻融法、载体沉积法等制备Cur/TMZ-LN,以包封率为评价指标选出最佳制备方法并进行单因素考查。结果:确定反复冻融法为最佳制备方法,且冻融次数为3,磷脂、胆固醇比为6∶1,磷脂浓度为3 mg/mL,姜黄素包封率为89.23%,替莫唑胺包封率为44.65%。结论:利用反复冻融法制备姜黄素/替莫唑胺脂质纳米粒,包封率较高,粒径较小。

Objective:Prepare lipid nanoparticles(Cur/TMZ-LN)co-loaded with curcumin(Cur)and temozolomide(TMZ).Methods:Cur/TMZ-LN was prepared by thin film dispersion,repeated freezing-thawing and carrier deposition.The best preparation method was selected based on the encapsulation efficiency.Results:The method of repeated freez-ing-thawing was confirmed,and the times of freezing-thawing were 3,the ratio of phospholipid to cholesterol was 6∶1,the concentration of phospholipid was 3 mg/mL,the entrapment efficiency of curcumin was 89.23%,the entrapment effi-ciency of temozolomide was 44.65%.Conclusion:Curcumin/temozolomide lipid nanoparticles were prepared by repeated freezing-thawing method with high entrapment efficiency and small particle size.