LC-MS/MS法测定大鼠呼吸道中的硫酸沙丁胺醇及其肺组织分布

Determination of Salbutamol Sulfate in Rat Respiratory Tract by LC-MS/MS and Its Lung Tissue Distribution

建立了LC-MS/MS法测定大鼠气管支气管、肺组织中的硫酸沙丁胺醇。结果显示,硫酸沙丁胺醇在大鼠气管支气管、肺中的线性范围分别为10.41~10410、5.205~5205 ng/g。不同组织样品的日内与日间RSD均小于15%。大鼠吸入给药后,硫酸沙丁胺醇在大鼠气管支气管及肺内呈现相似的动态变化过程,给药后即可达最大浓度,然后快速消除。吸入用硫酸沙丁胺醇溶液在靶部位气管支气管中的分布较多,为后续临床合理用药提供了参考依据。

The LC-MS/MS method was established to determine salbutamol sulfate in rat tracheobronchial and lung tissues.The results showed that the linear ranges in tracheobronchial and lung tissues were 10.41-10410 and 5.205–5205 ng/g,respectively.The intra-and inter-day RSDs of the samples from different tissues were less than 15%.After inhalation administration,salbutamol sulfate showed similar dynamic changes in the tracheobronchial and lung tissues of rats,and reached maximum concentration immediately after administration,followed by rapid elimination.The inhalation of salbutamol sulfate solution was widely distributed in the tracheal and bronchus of the target site,which provided a reference basis for the clinical use.