子宫内膜异位症患者血清炎性细胞因子水平与肠道菌群的相关性

Correlation between serum inflammatory cytokines and gut microbiota in patients with endometriosis

目的探究子宫内膜异位症(EMS)患者血清炎性细胞因子水平与肠道菌群的相关性,为该类患者的治疗提供参考。方法选择2020年4月至2023年4月来我院就诊的EMS患者400例作为EMS组以及同期来我院体检的健康者100例作为对照组。比较两组对象血清炎性细胞因子水平和肠道菌群数量。采用Pearson相关性分析EMS患者血清炎性细胞因子水平与肠道菌群关系。结果EMS组患者血清IL-1β、IL-17、TGF-β1、IL-22水平分别为(11.67±1.24)ng/L、(78.16±7.13)ng/L、(1.93±0.42)μg/L、(359.48±56.39)ng/L,均高于对照组的(4.05±0.98)ng/L、(52.02±6.58)ng/L、(1.51±0.34)μg/L、(203.54±57.11)ng/L(t=57.137、34.933、9.267、24.671,均P<0.05)。EMS组患者肠道肠杆菌、产气荚膜梭菌数量分别为(8.34±1.47)lg CFU/g、(8.69±1.32)lg CFU/g,均高于对照组的(5.36±1.18)lg CFU/g、(6.13±1.27)lg CFU/g(t=18.809、17.476,均P<0.05);而乳杆菌、双歧杆菌数量分别为(4.12±0.86)lg CFU/g、(7.11±1.32)lg CFU/g,均低于对照组的(7.41±1.05)lg CFU/g、(9.59±1.07)lg CFU/g(t=32.661、17.408,均P<0.05)。Pearson相关性分析显示,EMS患者IL-1β、IL-17、TGF-β1、IL-22水平与肠杆菌、产气荚膜梭菌数量均呈正相关(各血清炎症因子与肠杆菌r=0.621、0.675、0.589、0.637,各血清炎症因子与产气荚膜梭菌r=0.543、0.612、0.572、0.511;均P<0.05),而IL-1β、IL-17、TGF-β1、IL-22水平与乳杆菌、双歧杆菌数量均呈负相关(各血清炎症因子与乳杆菌r=−0.578、−0.509、−0.624、−0.582,各血清炎症因子与双歧杆菌r=−0.529、−0.517、−0.538、−0.546;均P<0.05)。结论EMS患者出现血清IL-1β、IL-17、TGF-β1、IL-22水平升高和肠道菌群紊乱情况,血清炎性细胞因子水平与肠道菌群存在一定关系,临床可考虑从改善机体炎症水平及肠道菌群来进行治疗。

Objective To explore the correlation between serum inflammatory cytokines and gut microbiota in patients with endometriosis(EMS),providing a reference for the treatment.Methods A total of 400 patients with EMS and 100 healthy controls in the hospital between April 2020 and April 2023 were enrolled as EMS group and control group,respectively.The levels of serum inflammatory cytokines and gut microbiota were compared between the two groups.The relationship between serum inflammatory cytokines and gut microbiota in EMS patients was analyzed using Pearson correlation analysis.Results The levels of serum IL-1β,IL-17,TGF-β1 and IL-22 in EMS group were(11.67±1.24)ng/L,(78.16±7.13)ng/L,(1.93±0.42)μg/L and(359.48±56.39)ng/L,higher than(4.05±0.98)ng/L,(52.02±6.58)ng/L,(1.51±0.34)μg/L and(203.54±57.11)ng/L in control group(t=57.137,34.933,9.267,24.671,all P<0.05).The counts of Enterobacter and Clostridium perfringens in EMS group were(8.34±1.47)lg CFU/g and(8.69±1.32)lg CFU/g,higher than(5.36±1.18)lg CFU/g and(6.13±1.27)lg CFU/g in control group(t=18.809,17.476;all P<0.05),while those of Lactobacillus and Bifidobacteria were(4.12±0.86)lg CFU/g and(7.11±1.32)lg CFU/g,lower than(7.41±1.05)lg CFU/g and(9.59±1.07)lg CFU/g in control group(t=32.661,17.408;all P<0.05).The levels of serum IL-1β,IL-17,TGF-β1 and IL-22 were positively correlated with counts of Enterobacter and Clostridium perfringens(inflammatory factor-Enterobacter:r=0.621,0.675,0.589,0.637;inflammatory factor-Clostridium perfringens:r=0.543,0.612,0.572,0.511;all P<0.05),while negatively correlated with counts of Lactobacillus and Bifidobacteria(inflammatory factor-Lactobacillus:r=−0.578,−0.509,−0.624,−0.582;serum inflammatory factor-Bifidobacteria:r=−0.529,−0.517,−0.538,−0.546;all P<0.05).Conclusion The levels of serum IL-1β,IL-17,TGF-β1 and IL-22 are increased,and gut microbiota are disordered in EMS patients.There is certain relationship between serum inflammatory cytokines and gut microbiota.Clinically,treatment can be performed based on the improvement of inflammation level and gut microbiota.