汽化过氧化氢在动物生物安全三级实验室终末消毒中的应用观察

Application of vaporized hydrogen peroxide in terminal disinfection in animal biosafety level 3 laboratory

目的探索汽化过氧化氢在动物生物安全三级实验室(ABSL-3实验室)终末消毒中对空间和风口型排风高效过滤器原位消毒的最优空间分布浓度和方案。方法分别采用5、8、10和15 g/m^(3)的过氧化氢对不同体积的空间进行消毒处理,通过化学指示卡和生物指示菌法对汽化过氧化氢灭菌效果进行验证,并观察其对室内彩钢板的腐蚀性;分别采用10、12和15 g/m^(3)的过氧化氢对排风高效过滤器进行原位消毒并筛选最佳空间分布浓度。结果10 g/m^(3)的汽化过氧化氢可有效对≤200 m^(3)的实验室空间进行消毒处理,对彩钢板无腐蚀。在消毒循环风机的辅助下,15 g/m^(3)的汽化过氧化氢可对实验室风口型排风高效过滤器进行原位有效消毒。结论汽化过氧化氢消毒机配合消毒循环风机的消毒方案可在ABSL-3实验室终末消毒时实现对房间和排风高效过滤器的同步消毒。

Objective To explore the optimal disinfection dosage and scheme of vaporized hydrogen peroxide on indoor air and high efficiency particulate air filter(HEPA)during terminal disinfection in animal biosafety level 3(ABSL-3)laboratory.Method The hydrogen peroxide at concentration of 5,8,10 and 15 g/m^(3)were used to disinfect different volumes of space,and the disinfection effect of vaporized hydrogen peroxide was verified by chemical indicator card and biological indicator bacteria,and its corrosiveness to indoor color steel plate was observed.Exhaust HEPA filters were disninfected in situ with 10,12 and 15 g/m^(3)of hydrogen peroxide which were screened for optimal dosage.Results The 10 g/m^(3)vaporized hydrogen peroxide could effectively disinfect the≤200 m^(3)laboratory space,and there was no corrosion of color steel plates.With the use of a disinfection circulating fan,15 g/m^(3)vaporized hydrogen peroxide could disinfect the laboratory tuyere-type exhaust HEPA filter in situ.Conclusion The disinfection scheme of vaporized hydrogen peroxide disinfection circulating fan can achieve the simultaneous disinfection of indoor air and exhaust HEPA filter during the termial disinfection of ABSL-3 laboratory.