针刺对高脂饮食诱导的肥胖小鼠炎症因子表达的影响

Effect of Acupuncture on the Expression of Inflammatory Factors in Obese Mice Induced by High Fat Diet

目的:观察针刺对高脂饮食诱导的肥胖小鼠的治疗作用及对血清炎症因子蛋白和m RNA表达的影响,探讨针刺减轻肥胖小鼠体质量的作用机制。方法:从50只C57BL/6J小鼠中随机筛选12只为空白组,饲以正常饮食,剩余38只饲以高脂饮食饲养12周。将造模成功的24只小鼠随机分为模型组和针刺组。治疗4周后,比较各组小鼠体质量、血清脂质水平的差异,HE染色观察各组小鼠肝脏和附睾脂肪的形态,用Western blotting检测各组小鼠血清中IL-1β、IL-6、TNF-α、MCP-1蛋白表达水平,采用实时荧光定量PCR检测各组小鼠血清中IL-1β m RNA、IL-6 mRNA、TNF-α mRNA、MCP-1 mRNA表达水平。结果:与模型组比较,针刺组小鼠体质量以及血清中TC、TG、LDL含量明显降低;针刺组小鼠的肝脏脂质代谢明显改善,脂肪细胞大小明显缩小,血清中炎性因子IL-1β、IL-6、TNF-α、MCP-1蛋白表达水平及IL-1β mRNA、IL-6 mRNA、TNF-α m RNA、MCP-1 mRNA的水平明显下降。结论:针刺能明显降低肥胖小鼠体质量,改善肥胖小鼠的病理损伤情况,下调血清中TC、TG、LDL的含量以及炎性因子IL-1β、IL-6、TNF-α、MCP-1蛋白表达水平及IL-1β mRNA、IL-6 mRNA、TNF-α mRNA、MCP-1 mRNA的表达,达到治疗肥胖的目的。

Objective:To observe the therapeutic effect of acupuncture on high-fat diet induced obese mice and its effect on changes in serum inflammatory factor protein and mRNA expression levels,and to explore the mechanism of acupuncture in reducing body mass in obese mice.Methods:Totally 12 C57BL/6J mice were randomly selected from 50 as the blank group,fed with a normal diet,and the remaining 38 mice were fed with a high-fat diet for 12 weeks.After successful modeling,24 mice were randomly divided into model group and acupuncture group.After 4 weeks of treatment,the differences in body weight and serum lipid levels of mice were compared among the 3 groups.The morphology of liver and epididymal fat in each group was observed by HE staining,and the expression of IL-1β,IL-6,TNF-αand MCP-1 protein were detected by Western blotting and the expression of IL-1βmRNA,IL-6 mRNA,TNF-αmRNA and MCP-1 mRNA were detected by real-time fluorescence quantitative PCR.Results:Compared with the model group,the body weight and the content of total cholesterol,triglycerides and low density lipoprotein were significantly reduced in the acupuncture group.The liver lipid metabolism was significantly improved in acupuncture group,and the size of adipocytes was significantly reduced in acupuncture group.The level of inflammatory factor,including IL-1β,IL-6,TNF-αand MCP-1,as well as the expression of IL-1βmRNA,IL-6 mRNA,TNF-αmRNA and MCP-1 mRNA decreased significantly in acupuncture group.Conclusion:Acupuncture can significantly reduce the weight of obese mice, improve their pathological damage, and down-regulate the levels of TC, TG and LDL in serum, the expression of inflammatory factor (IL-1β, IL-6,TNF-α and MCP-1 protein), and expression of IL-1β mRNA, IL-6 mRNA, TNF-α mRNA and MCP-1 mRNA, to achieve the goal of treating obesity.