RANKL对LPS诱导奶牛乳腺上皮细胞炎症反应的作用研究

Effect of RANKL on LPS-induced inflammatory response of cow mammary epithelial cells

文章研究利用LPS诱导建立奶牛乳腺上皮细胞炎症模型,过表达RANKL后,采用MTT法检测奶牛乳腺上皮细胞炎症模型中奶牛乳腺上皮细胞活力,采用荧光定量PCR技术检测奶牛乳腺上皮细胞炎症模型中炎症因子IL1β、IL6、i NOS、TNF-α的m RNA表达,探讨RANKL对LPS诱导的奶牛乳腺上皮细胞炎症反应的作用。结果表明,以10μg/mL LPS处理奶牛乳腺上皮细胞24 h建立奶牛乳腺上皮细胞炎症模型,RANKL可显著提高奶牛乳腺上皮细胞炎症模型中奶牛乳腺上皮细胞活力,降低奶牛乳腺上皮细胞炎症模型中IL1β、IL6、i NOS的mRNA表达,同时显著降低奶牛乳腺上皮细胞炎症模型中TNF-α的mRNA及蛋白表达。

In this study,an inflammation model of cow mammary epithelial cells was established by LPS induction.After RANKL was overexpressed,MTT assay was used to detect the activity of cow mammary epithelial cells in inflammatory models.And the mRNA expressions of inflammatory cytokines IL1β,IL6,iNOS and TNF-αin cow mammary epithelial cells were detected by fluorescence quantitative PCR.Further,the effect of RANKL on LPS-induced inflammatory response of cow mammary epithelial cells was investigated.The results showed that RANKL significantly increased the activity of mammary epithelial cells in the mammary epithelial cell inflammation model and significantly decreased the inflammatory cytokines IL1β,IL6,iNOS in the mammary epithelial cell inflammation model after the mammary epithelial cells were treated with 10μg/mL LPS for 24 h.In addition,the mRNA and protein expression of TNF-αin mammary epithelial cell inflammation models of were significantly decreased.