成都地区健康女性血清AMH参考区间的建立及与多种临床指标的相关性研究

Establishment of the reference intervals of serum AMH in healthy women in Chengdu area and itscorelation with various clinical indicators

目的:建立成都地区18~60岁健康女性血清抗苗勒管激素(AMH)的参考区间,并探讨年龄、血糖、血脂、尿酸(UA)、甲状腺激素等临床指标与AMH的相关性。方法:选取2020年8月至2023年7月在四川省人民医院健康管理中心进行健康体检的18~60岁女性作为研究对象,采集空腹静脉血,使用AMH定量检测试剂盒(ELISA)(广州市康润生物科技有限公司)测定AMH水平。经离群值判断,最终纳入1690例研究对象,按照年龄分为9组(18~22岁、23~25岁、26~30岁、31~35岁、36~40岁、41~45岁、46~50岁、51~55岁、56~60岁),建立成都地区健康女性血清AMH参考区间。使用多元线性回归分析年龄、血糖、血脂、UA、甲状腺激素等临床指标与AMH的相关性。结果:采用双侧限值(P_(2.5),P_(97.5))建立本地区健康女性AMH的参考区间,分别为18~22岁(2.74~9.37)ng/mL、23~25岁(1.28~14.55)ng/mL、26~30岁(0.98~16.50)ng/mL、31~35岁(0.38~12.13)ng/mL、36~40岁(0.10~7.70)ng/mL、41~45岁(0.06~4.35)ng/mL、46~50岁(0.06~1.19)ng/mL、51~55岁(0.06~0.35)ng/mL、56~60岁(0.06~0.08)ng/mL。以年龄为X轴,血清AMH水平为Y轴做散点图,可见血清AMH水平随年龄增加而降低。多元线性回归相关性分析显示,多种临床指标中,AMH与年龄呈负相关。结论:本研究建立了成都地区健康女性年龄特异性AMH的参考区间,为中国健康女性年龄特异性AMH的统一参考区间提供依据,进一步为AMH评估卵巢储备功能提供依据。

Objective:To establish the reference interval of serum anti-Mullerian hormone(AMH)in healthy women aged 18-60 years in Chengdu area,and to investigate the correlation between AMH and clinical indicators such as age,blood glucose,blood lipid,uric acid and thyroid hormone.Methods:Women aged 18-60 years who underwent physical examination in the Health Management Center of Sichuan Provincial People’s Hospital from August 2020 to July 2023 were selected as the study subjects.Fasting venous blood was collected,and AMH levels were determined by AMH quantitative detection kit(ELISA)(Guangzhou Kangrun Biotechnology Co.,LTD.).According to the outlier value,1690 subjects were finally included and divided into 9 groups according to age(18-22 years old,23-25 years old,26-30 years old,31-35 years old,36-40 years old,41-45 years old,46-50 years old,51-55 years old,56-60 years old)to establish the serum AMH reference interval of healthy women in Chengdu area.Multiple linear regression was used to analyze the correlation between AMH and age,blood glucose,blood lipids,uric acid,thyroid hormone and other clinical indicators.Results:Bilateral limits(P2.5,P97.5)were used to establish the reference intervals for AMH in healthy women in this area,which were 18-22 years old(2.74-9.37)ng/mL,23-25 years old(1.28-14.55)ng/mL,26-30 years old(0.98-16.50)ng/mL,31-35 years old(0.38-12.13)ng/mL,36-40 years old(0.10-7.70)ng/mL,41-45 years old(0.06-4.35)ng/mL,46-50 years old(0.06-1.19)ng/mL,51-55 years old(0.06-0.35)ng/mL,56-60 years old(0.06-0.08)ng/mL,respectively.Scatter plot with age as X-axis and serum AMH level as Y-axis showed that serum AMH level decreased with increasing age.Multiple linear regression correlation analysis showed that AMH was negatively correlated with age among various clinical indicators.Conclusion:Our study established a reference interval for age-specific AMH in healthy women in Chengdu area,It will provide a basis for a unified reference interval for age-specific AMH in healthy women in China,and further provide a basis for AMH assessment of ovarian reserve function.