TEAD1在小鼠急性心肌梗死后心肌组织巨噬细胞中的表达

The expression of TEAD1 in macrophages of myocardial tissue after acute myocardial infarction in mice

目的观察TEA转录因子1(TEAD1)在小鼠急性心肌梗死(AMI)后心肌组织中的表达情况及细胞定位,探讨TEAD1在急性心肌梗死后作用及相关机制。方法取40只6周龄C57BL/6小鼠随机分为急性心肌梗死组(AMI组)和假手术组(Sham组),术后以普通饲料喂养至1、3、7、14天分别处死并取材。本研究以HE染色及Masson染色观察急性心肌梗死后心肌组织的病理改变;免疫组化检测转录因子TEAD1和巨噬细胞标志物CD68在急性心肌梗死后小鼠心肌组织中的表达情况;免疫荧光双标法观察TEAD1在急性心肌梗死后心肌组织中是否在巨噬细胞中高表达。结果免疫组化结果显示,与Sham组相比,AMI组的TEAD1和CD68表达量显著增加(P<0.05),且主要在梗死交界区。AMI组中,TEAD1和CD68表达量随时间呈现先增加后减少的趋势,并在第3天达峰值,且相邻时间点间两者表达量差异均有统计学意义(P<0.05)。免疫荧光双标结果显示,AMI组巨噬细胞中TEAD1高表达,且除第14天外,相比于Sham组,AMI组表达TEAD1的巨噬细胞数量明显增加(P<0.05),并呈现先增加后减少的趋势,以第3天最多,相邻各时间点数量差异有统计学意义(P<0.05)。结论TEAD1和巨噬细胞标志物CD68在急性心肌梗死后心肌组织中高表达,且TEAD1可以定位在梗死交界区的巨噬细胞中。

Objective To observe the expression and cellular localization of TEA transcription factor 1(TEAD1)in myocardial tissue of mice after acute myocardial infarction(AMI),and to explore the role and mechanism of TEAD1 in AMI.Methods Forty C57BL/6 mice(6-weeks-old)were randomly divided into AMI group and Sham operation group.After operation,the mice were fed with common diet for 1,3,7 and 14 days and sacrificed.In this study,HE staining and Masson staining were used to observe the pathological changes of myocardial tissue after acute myocardial infarction.Immunohistochemistry was used to detect the expression of transcription factor TEAD1 and macrophage marker CD68 in myocardial tissue of mice after acute myocardial infarction.Immunofluorescence double-label method was used to observe whether TEAD1 was over-expressed in macrophages in myocardial tissue after acute myocardial infarction.Results Compared with the Sham group,the expression of TEAD1 and CD68 in AMI group was significantly increased(P<0.05),mainly in the infarction boundary area.In the AMI group,the expression of TEAD1 and CD68 showed a trend of first increasing and then decreasing over time,and reached the peak on the third day,and the differences between adjacent time points were statistically significant(P<0.05).The results of immunofluorescence double labeling showed that the expression of TEAD1 in AMI group was higher than that in Sham group(P<0.05)except on the 14th day,especially on the 3rd day,and showed a trend of first increasing and then decreasing.The quantity difference of adjacent time points was statistically significant(P<0.05).Conclusion TEAD1 and macrophage marker CD68 are highly expressed in myocardial tissue after acute myocardial infarction,and TEAD1 can be localized in macrophages at the infarction boundary.