基于16S rRNA测序技术分析圈养黑叶猴肠道微生物

Analysis of the gut microbiota of captive Trachypithecus francoisi based on 16S rRNA sequencing technology

研究旨在明确圈养黑叶猴(Trachypithecus francoisi)肠道菌群结构及功能,采用16S rRNA高通量测序技术对3只圈养成年个体(雄性记为M,雌性记为Fa和Fb)的粪便样品进行分析。结果显示,3只个体共注释到10个门,优势菌门为厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes),M个体厚壁菌门和螺旋体门(Spirochaetes)的相对丰度显著低于Fa和Fb个体(P<0.05),WPS-2相对丰度极显著高于Fa和Fb个体(P<0.01)。3只个体共注释到76个属,M个体毛螺菌科未分类属和f_鼠杆菌科_未分类属的相对丰度显著高于Fa和Fb个体(P<0.05),f_uncultured_rumen_bacterium_unclassified的相对丰度极显著高于Fa和Fb个体(P<0.01)。M个体瘤胃球菌UCG-005、密螺旋体科_2相对丰度显著低于Fa和Fb个体(P<0.05),瘤胃球菌UCG-002、瘤胃球菌UCG-013、f_瘤胃球菌_未分类属和瘤胃球菌UCG-014相对丰度极显著低于Fa和Fb个体(P<0.01)。M个体中未发现dgA-11_gut_group,Fa和Fb个体中未发现梭杆菌属(Fusobacterium)。KEGG分析发现,M个体新陈代谢通路富集基因相对丰度极显著高于Fa和Fb个体(P<0.01),但细胞过程通路富集基因相对丰度极显著低于Fa和Fb个体(P<0.01)。研究表明,圈养黑叶猴的雄性个体M与雌性个体Fa和Fb间肠道菌群组成及功能存在较大差异,可能主要与性别间的生理差异有关。

The study aimed to clarify the structure and function of the gut microbiota of captive Trachypithecus francoisi.High-throughput 16S rRNA sequencing technology was used to analyze fecal samples from three adult individuals(male denoted as M,and females as Fa and Fb).The results showed that a total of 10 phyla were annotated among the three individuals,with Firmicutes and Bacteroidetes being the dominant bacterial phyla.The relative abundances of Firmicutes and Spirochaetes in male individual M were significantly lower than those in females Fa and Fb(P<0.05),and the relative abundance of WPS-2 was extremely higher in M than in Fa and Fb(P<0.01).A total of 76 genera were annotated among the three individuals.The relative abundance of the unclassified family Lachnospiraceae and f_Ruminococcaceae_unclassified in male individual M was significantly higher than in Fa and Fb(P<0.05),and the relative abundances of f_uncultured_rumen_bacterium_unclassified were extremely higher in M than in Fa and Fb(P<0.01).The relative abundances of Ruminococcus UCG-005 and Treponema_2 in M were significantly lower than in Fa and Fb(P<0.05),and the relative abundances of Ruminococcus UCG-002,Ruminococcus UCG-013,f_Ruminococcaceae_unclassified,and Ruminococcus UCG-014 were extremely lower in M than in Fa and Fb(P<0.01).The dgA-11_gut_group was not found in M,and Fusobacterium was not found in Fa and Fb.KEGG analysis revealed that the relative abundance of metabolic pathway-enriched genes in M was extremely higher than in Fa and Fb(P<0.01),but the relative abundance of cell process pathway-enriched genes was extremely lower than in Fa and Fb(P<0.01).The study indicates that there are significant differences in the composition and function of the gut microbiota between male and female captive Trachypithecus francoisi,which may be mainly related to physiological differences between sexes.