微小RNA-151a-3p在非小细胞肺癌组织的表达及其与放疗敏感性的关系

Expression of microRNA-151a-3p in non-small cell lung cancer tissues and its relationship with radiotherapy sensitivity

目的探讨微小RNA(miR)-151a-3p在非小细胞肺组织中的表达及其与放疗敏感性的关系。方法选取2019年12月至2023年8月商丘市第一人民医院收治的54例非小细胞肺癌患者作为研究对象, 所有患者进行三维适形调强放射治疗, 评价患者放疗疗效, 分为完全缓解、部分缓解、稳定和进展。完全缓解和部分缓解为有效治疗组;稳定和进展为无效治疗组。分别采用荧光定量聚合酶链反应(PCR)分析有效治疗组和无效治疗组患者肿瘤组织miR-151a-3p表达水平, 根据miR-151a-3p均值, 分为高表达组和低表达组, 探讨miR-151a-3p表达水平与肺癌放疗敏感性的相关性。组间计量数据比较采用独立样本t检验。结果 54例患者, 其中22例完全缓解, 15例部分缓解, 10例稳定, 7例进展。完全缓解和部分缓解总计37例, 纳入有效治疗组, 稳定和进展17例患者纳入无效治疗组。放疗有效率为62.58%(37/54)。治疗有效组患者肿瘤组织miR-151a-3p表达水平(0.94±0.17)明显低于无效治疗组(1.49±0.29), 差异有统计学意义(t=8.807, P<0.05)。直径>5 cm和直径≤5 cm患者组三维适形调强放疗有效率[69.56%(16/23)、67.74%(21/31)]比较, 差异无统计学意义(χ2=1.008, P>0.05)。肺腺癌组患者和鳞状细胞癌组患者三维适形调强放疗有效率[65.0%(13/20)、70.59%(24/34)]比较, 差异无统计学意义(χ2=1.008, P>0.05)。低分化肿瘤患者组三维适形调强放疗有效率[87.5%(21/24)]明显高于中高分化肿瘤组放疗有效率[53.33%(16/30)], 差异有统计学意义(χ2=8.694, P<0.05)。淋巴结转移组患者三维适形调强放疗有效率[54.29% (19/35)]明显低于未淋巴结转移组患者[94.74%(18/19)], 差异有统计学意义(χ2=8.694, P<0.05)。miR-151a-3p低表达组患者三维适形调强放疗有效率[75.00%(30/40)]明显高于miR-151a-3p高表达组患者[50.00%(7/14)], 差异有统计学意义(χ2=8.694, P<0.05)。多因素Logistic回归分析显示, 低分化程度、无淋巴结转移、miR-151a-3p低表达水平是非小细胞肺癌三维适形调强放疗敏感性的预测因素[比值比(OR)=3.412、3.876、2.985, P<0.05]。相关性分析显示, 非小细胞肺癌组织中miR-151a-3p表达水平与三维适形调强放疗敏感性呈负相关(r=-0.479, P<0.05)。结论肺癌患者肿瘤组织中miR-151a-3p表达水平与放疗敏感性密切相关, 可用于临床放疗效果的评价。

ObjectiveTo investigate the expression of microRNA(miR)-151a-3p in non-small cell lung cancer(NSCLC)and its relationship with radiotherapy sensitivity.MethodsTotally,54 patients with NSCLC admitted to our hospital from December 2019 to August 2023 were selected as the study objects.All patients underwent three-dimensional conformal radiotherapy,and the radiotherapy efficacy of the patients was evaluated.The efficacy of patients was fallen into the following categories:complete response,partial response,stable and progressive.The patients obtaining the complete remission(n=22)and partial remission(n=15)served as the effective treatment group(n=37),and those obtaining stable response(n=10)and progressive response(n=7)as ineffective treatment group(n=17).The expression level of miR-151a-3p in tumor tissues in the effective treatment group and the ineffective treatment group was analyzed by fluorescence quantitative polymerase chain reaction(PCR).According to the mean expression level of miR-151a-3p,the patients were divided into miR-151a-3p high expression group and miR-151a-3p low expression group.The risk factors leading to lung cancer radiotherapy sensitivity were analyzed by multi-factor regression to explore the correlation between the expression level of miR-151a-3p and radiotherapy resistance of lung cancer.Independent samples t test was used to compare measurement data between groups.Results22 were in complete remission,15 were in partial remission,10 were stable and 7 were progressing.A total of 37 patients with complete and partial responses were included in the effective treatment group,and 17 patients with stable and progressive responses were included in the ineffective treatment group.The effective rate of radiotherapy was 62.58%(37/54).The expression level of miR-151a-3p in tumor tissues of the effective treatment group(0.94±0.17)was significantly lower than that of the ineffective treatment group(1.49±0.29,t=8.807,P<0.05).The effective rate of three-dimensional conformal intensity modulated radiotherapy in patients with poorly differentiated tumors[87.5%(21/24)]was significantly higher than that in radiotherapy for medium to high differentiated tumors[53.33%(16/30),χ2=8.694,P<0.05].The effective rate of three-dimensional conformal intensity modulated radiotherapy in patients with lymph node metastasis[54.29%(19/35)]was significantly lower than that in patients without lymph node metastasis[94.74%(18/19),χ2=8.694,P<0.05].The effective rate of three-dimensional conformal intensity modulated radiotherapy in patients with low expression of miR-151a-3p[75.00%(30/40)]was significantly higher than that in patients with high expression of miR-151a-3p[50.00%(7/14),χ2=8.694,P<0.05].Multivariate logistic regression analysis showed that low differentiation,no lymph node metastasis and low expression level of miR-151a-3p were predictive factors for the sensitivity of three-dimensional conformal intensity modulated radiotherapy in NSCLC[odds ratio(OR)=3.412,3.876,2.985,P<0.05].Correlation analysis showed that the expression level of miR-151a-3p in NSCLC tissue was negatively correlated with the sensitivity of three-dimensional conformal intensity modulated radiotherapy(r=-0.479,P<0.05).ConclusionMiR-151a-3p in tumor tissue of lung cancer patients is closely related to radiotherapy sensitivity,which can be used to evaluate clinical radiotherapy effect.