Chemerin、TNF-α在妊娠期糖尿病患者母血、脐血及脐带组织中的表达

Expression levels of Chemerin and TNF-α in peripheral blood,cord blood and umbilical cord tissue of gestational diabetes mellitus

目的测定Chemerin及肿瘤坏死因子(TNF-α)在妊娠期糖尿病(GDM)患者及胎儿中的表达水平,探讨Chemerin在GDM及胎儿发育中的作用。方法选择2016年8月至2017年11月产检并分娩的53例GDM孕妇为GDM组,选择同期产检并分娩的59例正常孕妇为正常妊娠组(NGT组)。酶联免疫吸附法测定2组患者血清中Chemerin及TNF-α的水平。实时荧光定量PCR和免疫印迹法测定脐带组织中Chemerin及TNF-αmRNA和蛋白的表达。结果2组母体血清Chemerin水平差异无统计学意义(P>0.05),GDM组脐血Chemerin明显高于NGT组(P<0.01)。2组母血Chemerin水平均明显低于脐血(P<0.01)。与NGT组母体血清中TNF-α水平相比,GDM组母体TNF-α明显升高(P<0.01)。2组脐血TNF-α水平差异无统计学意义(P>0.05)。GDM组母血TNF-α明显高于脐血(P<0.01),而NGT组母血TNF-α与脐血差异无统计学意义(P>0.05)。脐带组织中Chemerin mRNA在GDM组的表达水平明显高于NGT组(P<0.05),TNF-αmRNA在GDM组的表达水平明显高于NGT组(P<0.05)。Chemerin的蛋白表达在2组间差异无统计学意义(P>0.05),TNF-α蛋白在GDM组的表达明显高于NGT组(P<0.05)。相关性分析表明,GDM组中脐血血清Chemerin与TNF-α水平呈负相关(P<0.05);2组TNF-αmRNA及蛋白的表达均与新生儿体重呈正相关(P<0.05)。结论胎儿发育受到宫内炎症状态的调节,GDM患者脐血Chemerin可通过TNF-α间接发挥作用。

Objective To measure the expression levels of Chemerin and tumour necrosis factor alpha(TNF-α)in mothers with gestational diabetes mellitus(GDM)and the fetuses,and to clear the effect of Chemerin and TNF-α on the pathogeny of GDM and development of fetuses.Methods Fifty-three pregnant women with GDM who received prenatal examination and delivered from August 2016 to November 2017 were selected as the GDM group,and 59 healthy pregnant women who received prenatal examination and delivered during the same period selected as the normal pregnancy group(NGT group).Serum Chemerin and TNF-α levels in peripheral blood of pregnant women and cord blood were determined by enzyme-linked immunosorbent assay(ELISA).The mRNA and protein expressions of Chemerin and TNF-α in umbilical cord were determined by real-time quantitative PCR and Western blot,respectively.Results There was no significant difference in serum Chemerin level between GDM group and NGT group(P>0.05),while Chemerin level in cord blood was significantly higher than that of NGT group(P<0.01).Chemerin level in maternal blood was significantly lower than that of umbilical cord blood in both GDM group and NGT group(P<0.01).Compared with that of NGT group,serum TNF-α level in GDM group was significantly higher(P<0.01).There was no significant difference in cord blood TNF-α level between GDM group and NGT group(P>0.05).Maternal blood TNF-α level in the GDM group was significantly higher than that of cord blood level(P<0.01),but there was no significant difference between them in the NGT group(P>0.05).The mRNA expression of Chemerin in the GDM group was significantly higher than that of NGT group(P<0.05).The mRNA expression of TNF-α in the GDM group was significantly higher than that of NGT group(P<0.05).The protein expression of Chemerin was similar between the two groups(P>0.05).The protein expression of TNF-α in the GDM group was significantly higher than that of NGT group(P<0.05).The correlation analysis showed a negative correlation between cord blood levels of Chemerin and TNF-α in the GDM group(P<0.05).In both GDM group and NGT group,the mRNA and protein expressions of TNF-α were positively correlated with neonatal body weight(P<0.05).Conclusion Fetal development is regulated by intrauterine inflammation,and cord blood Chemerin in GDM patients can play an indirect role through TNF-α.