综合生物信息学分析紧密连接蛋白-11在乳腺癌中的作用

Comprehensive bioinformatics analysis of the role of claudin-11 in breast cancer

目的研究紧密连接蛋白-11(CLDN11)在乳腺癌中的表达水平及生存预后价值。方法首先使用UALCAN和KM-plotter数据库分析CLDN11在乳腺癌中的mRNA水平,然后分析其生存预后价值,最后使用bc-GenExMiner v4.5数据库分析CLDN11在不同临床参数乳腺癌中的表达水平。结果与正常组织比较,CLDN11 mRNA水平在乳腺癌中呈低表达(UALCAN数据库:P<0.001;KM-plotter数据库:P<0.001)。生存预后分析结果表明,低表达水平的CLDN11在乳腺癌中与更差的总生存期[UALCAN数据库:P=0.037;KM-plotter数据库:HR=0.58(0.42~0.80),Log-rank P<0.001]和疾病无进展生存期[KM-plotter数据库:HR=0.66(0.57~0.77),Log-rank P<0.001]相关。CLDN11 mRNA水平与不同年龄(P<0.001)、ER状态(P<0.001)、PR状态(P<0.001)、HER2状态(P<0.0001)、三阴型乳腺癌(TNBC)亚型(P=0.001)以及基底样及TNBC亚型(P<0.001)相关。结论CLDN11在乳腺癌中低表达,并且可以作为预后指标,但是其分子作用机制仍需要更多的实验研究证实。

Objective To investigate the expression level of claudin-11(CLDN11)in breast cancer and its prognostic value.Methods The mRNA levels of CLDN11 in breast cancer were analyzed using UALCAN and KM-Plotter databases.Secondly,its prognostic value was also analyzed.Finally,bc-GenExMiner V4.5 database was used to analyze the expression level of CLDN11 in breast cancer with different clinical parameters.Results CLDN11 mRNA levels were down-regulated in breast cancer compared to normal tissue(UALCAN database:P<0.001;KM-plotter database:P<0.001).Survival prognostic analysis showed that low expression of CLDN11 was associated with poorer overall survival[UALCAN database:P=0.037;KM-plotter database:HR=0.58(0.42~0.80),Log-rank P<0.001]and progression free survival[KM-plotter database:HR=0.66(0.57~0.77),Log-rank P<0.001]in breast cancer.CLDN11 mRNA level was correlated with different age(P<0.001),ER status(P<0.001),PR status(P<0.001),HER2 status(P<0.001),triple negative breast cancer(TNBC)subtype(P=0.001)and basal like TNBC subtype(P<0.001).Conclusion CLDN11 was down-regulated in breast cancer and can be used as a prognostic indicator.However,more experimental studies are needed to confirm the molecular mechanism.