脑动脉硬化患者血清miR-181b miR-126与内皮功能和炎症水平的相关性

Correlation of serum miR-181b and miR-126 with endothelial function and inflammation level in patients with cerebral arteriosclerosis

目的探讨脑动脉硬化患者血清miR-181b、miR-126与内皮功能和炎症水平的相关性。方法选取78例脑动脉硬化患者设为病例组,78名健康志愿者设为对照组。比较两组受试者血清miR-181b、miR-126表达,比较两组受试者血清一氧化氮、血管内皮素、促血管生成素-2、超敏C反应蛋白、白介素-6、白细胞计数水平。分析脑动脉硬化患者miR-181b、miR-126表达与内皮功能及炎症水平的相关性。结果病例组患者血清miR-181b、miR-126相对表达量均低于对照组(P<0.01)。病例组患者血清一氧化氮水平低于对照组,血清血管内皮素、促血管生成素-2、超敏C反应蛋白、白介素-6及白细胞水平均高于对照组(P<0.01)。脑动脉硬化患者miR-181b、miR-126均与血清一氧化氮水平呈正相关(P<0.01),与血清血管内皮素、促血管生成素-2、超敏C反应蛋白、白介素-6及白细胞水平呈负相关(P<0.01)。结论脑动脉硬化患者miR-181b、miR-126存在异常表达,且其与患者炎症水平及内皮功能具有显著相关性,可能通过调控炎症水平及内皮功能参与脑动脉硬化的发生发展。

Objective To explore the correlation of serum miR-181b(miR-181b)and miR-126(miR-126)of patients with cerebral arteriosclerosis(CAS)with endothelial function and inf lammation level.Methods Seventy-eight CAS patients were selected as case group and 78 healthy volunteers as control group.Such indexes were compared between two groups as serum miR-181b and miR-126 expression,so were the ser um levels of nitric oxide(NO),endothelin(ET),angiopoietin-2(Ang-2),hypersensitive C-reactive protein(hs-CRP),interleukin-6(IL-6)as well as white blood cell count(WBC).The cor relations of miR-181b and miR-126 expression with endot helial function and inflam mation level were analyzed.Results Relative expression levels of serum miR-181b and miR-126 were lower in case than control group(P<0.01).Serum NO level was lower and the levels of serum ET,Ang-2,hs-CR P,IL-6 and WBC were higher in case than control group(P<0.01).MicroR NA-181b and miR-126 of CAS patients were significantly positively related to NO level(P<0.01)and negatively to ET,Ang-2,hsCRP,IL-6 and WBC(P<0.01).Conclusion MicroRNA-181b and miR-126 are abnormally expressed in CAS patients and closely related to inflammation level and endothelial function significantly,which may be involved in the occurrence and development of cerebral arteriosclerosis via regulating inflammation level and endothelial function.