电子束辐照对酸枣仁中黄曲霉毒素及斯皮诺素含量的影响

Effects of Electron Beam Irradiation on Contents of Aflatoxin and Spinosin in Ziziphi Spinosae Semen

为明确电子束辐照对酸枣仁中黄曲霉毒素(AFB-1、AFB-2、AFG-1、AFG-2)的降解效果及斯皮诺素的影响。利用高效液相色谱法研究不同辐照剂量、不同含水量对酸枣仁中黄曲霉毒素(AFB-1、AFB-2、AFG-1、AFG-2)及斯皮诺素含量的影响。结果表明,乙腈溶液中AFB-1、AFB-2、AFG-1、AFG-2的降解率随辐照剂量的增加呈上升趋势;8 kGy辐照处理的AFB-1、AFB-2、AFG-1、AFG-2降解率最高,分别为97.25%、93.59%、75.11%、69.70%。随着辐照剂量和含水量的增加,酸枣仁中黄曲霉毒素的降解率呈上升趋势。含水量为23%的酸枣仁处理后黄曲霉毒素的降解率分别为30.58%、30.24%、38.04%、36.58%;经不同辐照剂量处理后的斯皮诺素的含量变化差异不显著(P>0.05),含水量为7%酸枣仁辐照处理后斯皮诺素的含量显著高于15%、19%、23%含水量的酸枣仁。因此,电子束辐照结合水分处理能有效降解酸枣仁中的黄曲霉毒素,然而水分含量过高不利于斯皮诺素含量的保持。

This study aimed to determine the effect of electron beam irradiation on the degradation of aflatoxins(AFB-1,AFB-2,AFG-1,AFG-2)and spinosin in Ziziphi Spinosae Semen.The effects of different irradiation doses and different water contents on the contents of aflatoxins(AFB-1,AFB-2,AFG-1,AFG-2)and spinosin in Ziziphi Spinosae Semen were investigated using high-performance liquid chromatography.The results showed that the degradation rates of AFB-1,AFB-2,AFG-1,and AFG-2 treated with acetonitrile solution increased with irradiation dose,and the degradation rates of AFB-1,AFB-2,AFG-1,and AFG-2 treated with 8 kGy irradiation were 97.25%,93.59%,75.11%,and 69.70%,respectively.With the increase of irradiation dose and water content,the degradation rate of aflatoxin in Ziziphi Spinosae Semen also increased.The aflatoxin degradation rates of Ziziphi Spinosae Semen with 23% water content were 30.58%,30.24%,38.04%,and 36.58%,respectively.There was no significant difference in the content of spinosin after different irradiation doses(P>0.05).The content of spinosin in Ziziphi Spinosae Semen irradiated with 7% water content was significantly higher than that in Ziziphi Spinosae Semen with water contents of 15%,19%,and 23%.In conclusion,electron beam irradiation combined with water treatment can effectively degrade aflatoxin in Ziziphi Spinosae Semen;however,too high water content is not conducive to the maintenance of spinosin content.