The clinical differentiation of blood culture-positive and -negative sepsis in burn patients: a retrospective cohort stu

Background:Sepsis is a potentially life-threatening condition that occurs when the body’s response to infection leads to widespread inflammation and tissue damage.Negative cultures can make it difficult for clinicians to make a diagnosis and may raise questions about the validity of the definition of sepsis.In addition,the clinical distinctions between burn patients with blood culturepositive and-negative sepsis are also poorly understood.Therefore,this study aimed to examine the clinical differences between blood culture-positive and-negative sepsis in burn patients in order to improve the understanding of the pathophysiology and epidemiology of sepsis in this population.Methods:This study had a retrospective design,and the participants were adults aged≥18 years.Patients diagnosed with sepsis were divided into two groups based on their blood culture results within 1 week of sepsis diagnosis.Results:We enrolled 1643 patients admitted to our institution’s burn intensive care unit between January 2010 and December 2021.pH,platelet count,bicarbonate and haematocrit were significant in both the positive and negative groups.However,lymphocyte,red cell distribution width and blood urea nitrogen were significant only in the positive group,whereas lactate dehydrogenase was significant only in the negative group.Acinetobacter baumannii,Pseudomonas aeruginosa,and Klebsiella pneumonia are common gram-negative bacterial species,and Staphylococcus aureus and Staphylococcus epidermidis are common gram-positive bacterial species seen in burn patients with positive blood cultures.Carbapenem resistance was found to be associated with an unfavourable prognosis in gram-negative bacteria,with the exception of P.aeruginosa.Conclusions:pH,platelet count,bicarbonate and haematocrit were routine biomarkers that demon-strated statistical significance in both groups.Lactate dehydrogenase was significant in the blood-negative group,while red cell distribution width,blood urea nitrogen and lymphocyte count were significant in the positive group.Furthermore,the most common causes of sepsis are gram-negative bacteria,including A.baumannii,K.pneumoniae and P.aeruginosa.Additionally,resistance to carbapenems is associated with unfavourable outcomes.