Atligator Web:A Graphical User Interface for Analysis and Design of Protein-Peptide Interactions

A key functionality of proteins is based on their ability to form interactions with other proteins or peptides.These interactions are neither easily described nor fully understood,which is why the design of specific protein–protein interaction interfaces remains a challenge for protein engineering.We recently developed the software ATLIGATOR to extract common interaction patterns between different types of amino acids and store them in a database.The tool enables the user to better understand frequent interaction patterns and find groups of interactions.Furthermore,frequent motifs can be directly transferred from the database to a user-defined scaffold as a starting point for the engineering of new binding capabilities.Since three-dimensional visualization is a crucial part of ATLIGATOR,we created ATLIGATOR web—a web server offering an intuitive graphical user interface(GUI)available at https://atligator.uni-bayreuth.de.This new interface empowers users to apply ATLIGATOR by providing easy access with having all parts directly connected.Moreover,we extended the web by a design functionality so that,overall,ATLIGATOR web facilitates the use of ATLIGATOR with a more intuitive UI and advanced design options.