摘要
Microbes well-adapted to the Arctic Ocean are promising for producing novel compounds,due to their fancy strategies for adaptation and being under-investigated.Two new phenazine alkaloids(1 and 2)and one new phenoxa-zine(3)were isolated from Nocardiopsis dassonvillei 502F,a strain originally isolated from Arctic deep-sea sediments.AntiSMASH analysis of the genome of Nocardiopsis dassonvillei 502F revealed the presence of 16 putative biosynthetic gene clusters(BGCs),including a phenazine BGC.Most of the isolated compounds were evaluated for their antibacterial,antiallergic,and cytotoxic activities.Among them,compounds 4 and 5 exhibited potent in vitro cytotoxic activities against osteosarcoma cell line 143B with IC_(50) values 0.16 and 20.0μM,respectively.Besides,the results of antiallergic activities of compounds 6-8 exhibited inhibitory activities with IC_(50) values of 10.88±3.05,38.88±3.29,and 2.44±0.17μg/mL,respectively(IC_(50)91.6μM for the positive control loratadine).
基金
the Guangdong Basic and Applied Basic Research Foundation(2021A1515011523,2021B1515120046,2022B1515120075)
National Key Research and Development Program of China(2022YFC2807501)
National Natural Science Foundation of China(grant no.41976224)
Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City(2021JJLH0097,2021CXLH0013)
Hainan Provincial Natural Science Foundation of China(823CXTD393)。
