广西壮族自治区百色市人群miR-145基因多态性分布特征及其与血脂的关系

Distribution characteristics of miR-145 gene polymorphism and its relationship with blood lipid in Baise city,Guangxi Zhuang Autonomous Region

目的探讨广西壮族自治区百色市人群微小RNA-145(miR-145)基因rs41291957、rs353291多态性分布特点,比较其基因型及等位基因频率与其他种族的分布差异,进一步分析rs41291957、rs353291多态性与血脂的关系。方法选取2020年7月至2021年12月在百色市人民医院保健科进行健康体检并进行miR-145基因检测的275名健康者作为研究对象。采用TaqMan探针法单核苷酸多态性分型技术检测rs41291957、rs353291的基因型及基因频率,使用TBA-FX8 Canon全自动生化仪检测血脂相关指标。通过统计学方法分别比较rs41291957、rs353291多态性在不同性别、种族分布中的差异,以及不同基因型血脂水平的差异。结果275名研究对象中miR-145基因rs41291957、rs353291存在AA、AG、GG 3种基因型,两位点基因型频率分布符合Hardy-Weinberg遗传平衡定律,差异无统计学意义(P>0.05),具有群体代表性。不同性别研究对象rs41291957、rs353291多态性比较,差异均无统计学意义(P>0.05)。275名研究对象rs41291957、rs353291位点多态性分布与东亚人群,以及rs353291位点多态性分布与欧洲人群比较,差异均无统计学意义(P>0.05)。275名研究对象rs41291957位点多态性分布与欧洲人群,以及rs41291957、rs353291位点多态性分布与非洲、美国、南亚人群比较,差异均有统计学意义(P<0.05)。rs353291位点3种基因型各项血脂水平,以及rs41291957位点3种基因型总胆固醇、甘油三酯、低密度脂蛋白胆固醇比较,差异均无统计学意义(P>0.05);rs41291957位点3种基因型高密度脂蛋白胆固醇比较,差异均有统计学意义(P<0.05)。结论广西壮族自治区百色市人群miR-145基因rs41291957、rs353291多态性分布不受性别的影响,但在不同种族之间的分布情况存在差异;miR-145基因rs41291957多态性与高密度脂蛋白胆固醇水平密切相关。

Objective To investigate the distribution characteristics of microRNA-145(miR-145)gene rs41291957 and rs353291 polymorphisms in Baise city,Guangxi Zhuang Autonomous Region,and to compare their genotype and allele frequencies with other ethnic groups.The distribution differences were further analyzed to analyze the relationship between rs41291957 and rs353291 polymorphisms and blood lipids.Methods A total of 275 healthy people who underwent physical examination and miR-145 gene detection in Health Care Department of Baise People′s Hospital from July 2020 to December 2021 were selected as the research objects.The genotypes and gene frequencies of rs41291957 and rs353291 were detected by TaqMan probe single nucleotide polymorphism typing technique,and the blood lipid related indexes were detected by TBA-FX8 Canon automatic biochemical analyzer.The differences in gender and ethnic distribution of rs41291957 and rs353291 polymorphisms,as well as the differences in blood lipid levels of different genotypes were compared by statistical methods.Results There were three genotypes of AA,AG and GG in miR-145 gene rs41291957 and rs353291 in 275 subjects.The genotype frequency distribution of the two sites conformed to the Hardy-Weinberg genetic equilibrium law,and the differences were not statistically significant(P>0.05),which was representative of the population.Comparison of rs41291957 and rs353291 polymorphisms between different gender subjects,and the differences were not statistically significant(P>0.05).There were no significant difference in the distribution of rs41291957 and rs353291 polymorphisms between 275 subjects and East Asian population,and the distribution of rs353291 polymorphisms between 275 subjects and European population(P>0.05).The distribution of rs41291957 locus polymorphism in 275 subjects was significantly different from that in European population,and the distribution of rs41291957 and rs353291 locus polymorphism was significantly different from that in African,American and South Asian populations(P<0.05).There were no significant differences in blood lipid levels among the subjects with three genotypes of rs353291 locus,and there were no significant differences in total cholesterol,triglyceride and low density lipoprotein cholesterol among the subjects with three genotypes of rs41291957 locus(P>0.05).There were significant differences in high-density lipoprotein cholesterol among the three genotypes of rs41291957(P<0.05).Conclusion The distribution of miR-145 gene rs41291957 and rs353291 polymorphisms in Baise city,Guangxi Zhuang Autonomous Region is not affected by gender,but there are differences in the distribution between different races.The polymorphism of miR-145 gene rs41291957 is closely related to the level of high density lipoprotein cholesterol.