心火脾虚证型特应性皮炎患者汗液差异代谢物及相关通路分析

Analysis of Differential Sweat Metabolites and Related Pathways in Patients with Atopic Dermatitis Characterized by Heart Fire and Spleen Deficiency Syndrome

目的探究心火脾虚证型特应性皮炎(AD)患者汗液差异代谢物与相关通路。方法招募心火脾虚证型特应性皮炎患者与健康志愿者各22例。对所收集的汗液标本进行广泛靶向代谢组学检测,通过多元统计分析筛选出差异代谢物。利用京都基因与基因组百科全书(KEGG)数据库、人类代谢组数据库(HMDB)对差异代谢物进行相关通路与关联疾病分析。结果在两组汗液标本间,共筛选出115个差异代谢物。经KEGG数据库注释分析,共涉及15个通路。HMDB关联疾病分析结果提示与消化系统疾病关系密切,包括结直肠癌、嗜酸性食管炎、克罗恩病和溃疡性结肠炎。结论心火脾虚证型特应性皮炎患者与健康对照组间存在众多差异代谢物,可为共病临床早期预警提供线索。

Objective To investigate the differential sweat metabolites and related pathways in patients with atopic dermatitis(AD)characterized by heart fire and spleen deficiency syndrome.Methods Twenty-two AD patients with heart fire and spleen deficiency syndrome and twenty-two 22 healthy volunteers were recruited.Extensive targeted metabolomics analysis was conducted on collected sweat samples,and differential metabolites were identified through multivariate statistical analysis.The KEGG databases and HMDB were utilized to analyze the related pathways and associated diseases of the differential metabolites.Results A total of 115 differential metabolites were identified between the two groups of sweat samples.According to the KEGG database annotation analysis,these metabolites were involved in 15 different pathways.The HMDB′s disease association analysis suggested a close relationship with digestive system diseases,including colorectal cancer,eosinophilic esophagitis,Crohn′s disease,and ulcerative colitis.Conclusion There are numerous differential metabolites between AD patients with heart fire and spleen deficiency syndrome and the healthy control group,which may provide clues for the early warning of comorbidities in clinical practice.