类风湿性关节炎患者血清抗环瓜氨酸肽抗体检测方法的对比分析

Comparative Analysis of Serum Anti-Cyclic Citrullinated Peptide Antibody Detection Methods in Patients with Rheumatoid Arthritis

目的分析研究类风湿性关节炎患者血清抗环瓜氨酸肽抗体检测方法的对比。方法选取2022年5月至2023年11月新乡市中心医院类风湿关节炎的90例患者为研究对象,所有人均行血清抗环瓜氨酸肽抗体检测,依次采用酶联免疫法、免疫胶体金法、免疫透射比浊法和化学发光法检测,比较各种检测方式与酶联免疫法检测结果的符合率。评价符合率较高的检测方法的精准性。结果免疫胶体金法、免疫透射比浊法、化学发光法与酶联免疫法的阳性符合率分别为:94.44%、88.89%、100%;总体符合率分别为:86.67%、83.33%、97.78%;Kappa值分别为:0.39、0.61、0.86。化学发光法仪器温度波动不超过1℃,发光重复率不超过5%,发光稳定性变化不超过±5%,精密度变化不超过8%,化学发光法检测诊断有较高的精准度。结论化学发光法与酶联免疫法有良好的结果一致性,化学发光法的精密度较高符合临床诊断要求,值得作为初筛血清抗环瓜氨酸肽抗体的首选方式,可以提高诊断准确率。

Objective To analyze and compare the detection methods of serum anti-cyclic citrullinated peptide antibodies in patients with rheumatoid arthritis.Methods A total of 90 RA patients treated at Xinxiang Central Hospital from May 2022 to November 2023 were selected as research subjects.All patients underwent serum anti-CCP antibody detection using enzyme-linked immunosorbent assay,immunocolloidal gold method,immunoturbidimetric method,and chemiluminescence method.The concordance rates of various detection methods with enzyme-linked immunosorbent assay were compared,and the precision of the detection methods with higher concordance rates was evaluated.Results The positive concordance rates of the immunocolloidal gold method,immunoturbidimetric method,and chemiluminescence method with enzyme-linked immunosorbent assay were 94.44%,88.89%,and 100%,respectively.The overall concordance rates were 86.67%,83.33%,and 97.78%,respectively.The Kappa values were 0.39,0.61,and 0.86,respectively.The temperature fluctuation of the chemiluminescence instrument did not exceed 1°C,the repeatability of luminescence did not exceed 5%,the stability change of luminescence did not exceed±5%,and the precision change did not exceed 8%.The chemiluminescence method had high diagnostic accuracy.Conclusion The chemiluminescence method has good consistency with enzyme-linked immunosorbent assay and high precision,meeting clinical diagnostic requirements.It is worth considering as the preferred method for initial screening of serum anti-CCP antibodies to improve diagnostic accuracy.