杏仁皮缩合单宁酶法提取工艺优化

Optimization of extraction process for condensed tannins from almond skin by enzyme method

在单因素基础上,利用响应面法优化杏仁皮缩合单宁的酶法提取工艺。以杏仁皮缩合单宁提取量为指标,考察了酶的用量、溶液pH值、提取温度、提取时间对杏仁皮缩合单宁提取量的影响。结果显示,所建立的杏仁皮缩合单宁提取工艺模型极显著,且在酶用量为26 U/g、溶液pH值为4.2、提取温度为52℃、提取时间为4 h的条件下,杏仁皮缩合单宁的提取量最高,预测值为38.71 mg/g,实际值为38.73 mg/g,结果表明优化的最佳提取工艺与预测方法相符。

On the basis of single factor analysis,the enzymatic extraction process of condensed tannins from almond skin was optimized using response surface methodology.The effects of enzyme amount,solution pH,extraction temperature,and extraction time on the extraction amount of condensed tannins from almond skin were investigated using almond skin as an indicator.The results showed that the established process model for extracting condensed tannins from almond skin was extremely significant,and under the conditions of enzyme amount of 26 U/g,solution pH of 4.2,extraction temperature of 52℃,and extraction time of 4 h,the highest extraction amount of condensed tannins from almond skin was found,with a predicted value of 38.71 mg/g and an actual value of 38.73 mg/g.This indicates that the optimal extraction process for condensed tannins from almond skin using enzyme method was successfully established.