高效液相色谱法测定土壤中的三种有机酸

Determination of Three Organic Acids in Soil by High Performance Liquid Chromatography

实验选取不同类型的土壤样品,考察并优化确定了提取方法、提取时长、提取温度和提取液固比,建立了高效液相色谱仪测定土壤中有机酸的检测方法。研究表明,高效液相色谱法(HPLC)检测有机酸的线性相关系数均达到0.999以上,线性良好。按照提取液固比5∶1在室温条件下水平振荡提取30 min,滤液用液相色谱法测定。土壤种有机酸加标回收率为97.3%~106.0%,检测结果相对偏差为1.2%~7.0%。结果结果表明,该方法具有精密度好、简便、环保、准确度高且成本低廉等优点,适用于土壤中有机酸的批量检测。

Different types of soil samples were used in this experiment,the extraction method,extraction time,extraction temperature and extraction liquid-solid ratio were investigated and optimized,and the detection method of organic acids in soil by high performance liquid chromatography(HPLC)was established.The results show that the linear correlation coefficients of this method are above 0.999,and the linearity is good.According to the extraction liquid-solid ratio of 5∶1,horizontal oscillation at room temperature for 30 minutes,and the filtrate is determined by liquid chromatography.The recovery rate of organic acids in soil was 97.3%-106.0%,and the relative deviation of the detection results was 1.2%-7.0%.The results showed that the method had high accuracy and good precision.The method has the advantages of simplicity,environmental protection,high accuracy and low cost,and is suitable for the batch detection of organic acids in soil.