筇竹K^(+)通道蛋白QtKCO1基因的克隆与表达分析

Cloning and Expression Analysis on QtKCO1 Gene from Qiongzhuea tumidinoda

为分析KCO家族基因在筇竹钾运输中起到的重要作用,本研究以筇竹实生苗(2年生)为试材,利用RACE技术,克隆出KCO家族基因,命名为QtKCO1(基因登录号:MT078981),并分析其表达特征。结果表明,QtKCO1基因全长为1502 bp,开放阅读框为1041 bp,编码347个氨基酸。QtKCO1具有5个跨膜区域,且与野生二粒小麦(Triticum dicoccoides)的同源性最高,为85.88%。另外,QtKCO1基因是组成型表达,在筇竹每个组织器官(竹笋,根,茎,叶)均有表达,表达量依次为竹笋>茎>叶>根。高浓度KCl胁迫下,随着处理时间的增加,QtKCO1基因在根表达量增加,而在茎和叶中表达量则下降。与对照组相比,NaCl胁迫处理条件下,筇竹QtKCO1在筇竹各个组织器官中均呈现上调表达,且表达量依次为叶>茎>根。本研究为进一步分析KCO家族基因在钾运输中的作用提供了理论依据。

In order to study the important role of KCO family genes in potassium transport of Qiongzhuea tumidinoda,the KCO gene was cloned from the 2-year-old seedlings of Qiongzhuea tumidinoda by RACE technique.The gene was named QtKCO1(GenBank number:MT078981)and its expression characteristics were analyzed.The results showed that the full length of QtKCO1 gene was 1502 bp,and the open reading frame(ORF)of QtKCO1 was 1041 bp,encoding 347 amino acids.QtKCO1 had five transmembrane regions and showed the highest homology of 85.88%with wild two-grain wheat(Triticum dicoccoides).expressed compositionally in all tissues and organs(bamboo shoots,roots,stems,le aves),and the expression levels were in the order of bamboo shoots>stems>leaves>roots.Under high concentration KCl stress,the expression of QtKCO1 gene increased in roots,but decreased in stems and leaves with the increase of treatment time.Compared with the control group,QtKCO1 expression was up-regulated in all tissues and organs of Qiongzhuea tumidinoda under NaCl stress,and the expression level was in the order of leaves>stems>roots.This study provides a theoretical basis for further analysis of the role of KCO family genes in potassium transport.