十字花科植物抗氧化基因(OXR)的鉴定与生物信息学分析

Identification and Bioinformatics Analysis of Antioxidant Genes(OXR)in Cruciferous Plants

OXR(Oxidation resistance)基因含有TLDc保守结构域,能够有效清除活性氧,在细胞抗氧化损伤中起着重要的作用。本研究基于小盐芥(Thellungiella halophila,Tha)、白菜(Brassica rapa,Bra)、甘蓝(Brassica oleracea,Bol)、黑芥(Brassica nigra,Bni)、甘蓝型油菜(Brassica napus,Bna)、芥菜型油菜(Brassica juncea,Bju)和胡萝卜(Daucus carota,Dca)7种十字花科植物基因组数据库,通过与拟南芥(Arabidopsis thaliana,At)的6个OXR基因序列比对,共筛选鉴定出所选植物的50个OXR基因家族成员,并对其进行生物信息学分析。系统进化树的构建表明,OXR基因家族成员有5个亚分支;顺式作用元件分析显示,OXR基因启动子区域含有多个响应温度和激素的顺式作用元件和结合位点,表明十字花科植物的OXR基因家族成员可能参与逆境响应和激素调节;OXR基因在染色体上分布不均匀,并有24对OXR串联重复基因,表明OXR基因具有高度保守性。本研究为进一步揭示植物OXR基因的功能提供了依据。

OXR(oxidation resistance)gene contains the TLDc conserved structural domain,which can effectively remove reactive oxygen and plays an important role in cell antioxidant damage.Based on the genomic database of seven cruciferous plants(Thellungiella halophila,Tha;Brassica rapa,Bra;Brassica oleracea,Bol;Brassica nigra,Bni;Brassica napus,Bna;Brassica juncea,Bju and Daucus carota,Dca),50 OXR gene family members of selected plants were screened and identified by comparing with six OXR gene sequences of A rabidopsis thaliana(At),and bioinformatics analysis of them was carried out.The phylogenetic tree showed that the OXR gene family members had five subbranches.Cis-acting element analysis showed that the OXR gene promoter region contained multiple cis-acting elements and binding sites in response to temperature stress and hormones,indicating that members of the OXR gene family of cruciferous plants may be involved in stress response and hormone regulation.The OXR genes were not equally distributed on chromosomes,and there were 24 pairs of OXR genes distributed on chromosomes with tandem repeats,indicating that the OXR genes were highly conserved.This study provides a basis for further research on OXR genes in plant.