拟南芥和番茄斑萎病毒NSs蛋白互作的转录组分析

Transcriptomic Analysis of Interaction between A rabidopsis thaliana and NSs Protein of Tomato Spotted Wilt Tospovirus

为了研究番茄斑萎病毒NSs蛋白在番茄斑萎病毒与植物互作中发挥的作用,本研究以转NSs基因拟南芥和生态型拟南芥为研究对象,使用Illumina RNA-seq测序平台进行转录组测序,利用生物信息学方法分析NSs对植物转录水平影响。结果显示,转基因和生态型拟南芥共有1258个差异表达基因。其中966个差异表达基因富集到Gene Ontology(GO),主要涉及细胞组分、代谢过程和酶活性等功能。517个差异表达基因富集到KEGG通路中,NSs主要降低了苯丙烷生物合成、植物激素信号转导、植物病原体相互作用等通路基因的表达,这些通路都与植物防御病原微生物侵染相关。101个差异表达基因被鉴定为转录因子,分属于39个转录因子家族,包括调控苯丙烷生物合成的MYB家族和调控生长素信号转导AUX/IAA转录因子家族。研究发现,NSs蛋白与拟南芥的互作是一个复杂的过程,NSs影响了拟南芥的次生代谢、激素调节、防御反应等多项生物功能,这为后续深入研究NSs在番茄斑萎病毒与宿主植物互作中发挥的作用以及番茄斑萎病毒的防治提供参考依据。

In order to study the role of NSs protein in the interaction mechanism between tomato spotted wilt tospovirus and plants,this study took transgenic A rabidopsis thaliana expressing NSs gene and wild-type A rabidopsis thaliana as experimental materials,and used the Illumina RNA-seq sequencing platform for transcriptome sequencing,and analyzed the effects of NSs on gene expression and function by bioinformatics methods.The results showed that there were 1258 differentially expressed genes(DEGs)between transgenic and wild-type A rabidopsis.Among them,966 DEGs were enriched in Gene Ontology(GO).Their functions were mainly referred to cell components,metabolic processes and enzyme activities.517 DEGs were enriched in KEGG pathway,it was showed that NSs reduced the expression of genes in the pathways such as phenylpropanoid biosynthesis,plant hormone signal transduction,and plant-pathogen interaction.These pathways are all related to the defense of plants against pathogenic microorganisms.101 DEGs were identified as transcription factors,belonging to 39 transcription factor families,including the MYB family that regulates phenylpropanoid biosynthesis and the AUX/IAA transcription factor family that regulates auxin signal transduction.Through the study,it is found that the interaction between NSs protein and Arabidopsis is a complicated process.NSs influences the secondary metabolism,hormone regulation,defense response and other biological functions of Arabidopsis.This lays the foundation for the in-depth study of the role of NSs in the interaction between tomato spotted wilt tospovirus and host plants and the control of tomato spotted wilt tospovirus.