基于ISSR分子标记的花叶藓科植物遗传多样性分析

Genetic Diversity Analysis of Calymperaceae Based on ISSR Molecular Marker

为了解花叶藓科内植物的遗传多样性,本研究采用ISSR分子标记对采集于海南省和广东省的16种花叶藓科植物的遗传多样性和亲缘关系进行分析。结果显示:(1)筛选出的8条ISSR引物共扩增出201条条带,其中多态性的条带有118条,多态性比例为58.71%,平均每个引物扩增出14.75个多态性条带。(2)16种花叶藓科植物的遗传相似系数范围为0.4925~0.8109,平均遗传相似系数为0.6527。(3)将0.6900作为最低相似系数时,16种花叶藓科植物被分为7大类:第一类包括梯网花叶藓(Calymperes afzelii)、兜叶花叶藓(Calymperes moluccense)、匍网藓(Mitthyridium fasciculatum)、齿边花叶藓(Calymperes serratum)、圆网花叶藓(Calymperes erosum);第二类包括黄匍网藓(Mitthyridium flavum)、细刺网藓(Syrrhopodon spiculosus)、鞘刺网藓(Syrrhopodon armatus)、八齿藓(Octoblepharum albidum)、刺肋白睫藓(Leucophanes albescens);第三类包括网藓(Syrrhopodon gardneri);第四类包括巴西网藓原变种(Syrrhopodon prolifer var.prolifer);第五类包括巴西网藓鞘齿变种(Syrrhopodon prolifer var.tosaensis)、卷叶网藓(Syrrhopodon involutus);第六类包括日本网藓(Syrrhopodon japonicus);第七类包括拟兜叶花叶藓(Calymperes graeffeanum)。本研究从分子水平上揭示了花叶藓科植物的遗传多样性和亲缘关系,为花叶藓科植物的分类学地位提供了理论依据。

In this study,to understand the genetic diversity of Calymperaceae plants,ISSR molecular markers were used to analyze the genetic diversity and genetic relationships of 16 Calymperaceae plants collected in Hainan and Guangdong provinces.The results showed that:(1)A total of 201 bands were amplified from the 8 ISSR primers selected,among them,118 were polymorphic bands with a polymorphism ratio of 58.71%,on average,14.75 polymorphism bands were amplified per primer.(2)The genetic similarity coefficient of 16 species of Calymperaceae was in the range of 0.4925~0.8109,and the average genetic similarity coefficient was 0.6527.(3)When 0.6900 was used as the lowest similarity coefficient,the 16 species of Calymperaceae were divided into 7 categories:The first category includes Calymperes afzelii,Calymperes moluccense,Mitthyridium fasciculatum,Calymperes serratum,Calymperes erosum;the second category includes Mitthyridium flavum,Syrrhopodon spiculosus,Syrrhopodon armatus,Octoblepharum albidum,Leucophanes albescens;the third category includes Syrrhopodon gardneri;the fourth category includes Syrrhopodon prolifer var.prolifer;the fifth category includes Syrrhopodon prolifer var.tosaensis,Syrrhopodon involutus;the sixth category includes Syrrhopodon japonicus;the seventh category includes Calymperes graeffeanum.This study reveals the genetic diversity andgenetic relationships of Calymperaceae at the molecular level,and provides a theoretical basis for the taxonomic status of Calymperaceae.