脑源性神经营养因子在轻型子宫内膜异位症中的表达及意义

Expression and significance of brain-derived neurotrophic factor in mild endometriosis

目的:探讨脑源性神经营养因子(BDNF)在轻型子宫内膜异位症中的表达,评估BDNF作为轻度子宫内膜异位症临床标志物的适用性及诊断价值。方法:选取2020~2022年中山市博爱医院收治的因子宫内膜异位症及输卵管性不孕就诊的行腹腔镜手术的患者159例为研究对象,分为研究组(腹腔镜及病理证实的子宫内膜异位症患者,分为轻型子宫内膜异位症与重型子宫内膜异位症)111例与对照组(输卵管性不孕患者)48例。术前收集所有患者的外周血,通过酶联免疫吸附试验(ELISA)方法定量检测BDNF及CA125水平。比较研究组与对照组血清BDNF及CA125水平;比较轻型子宫内膜异位症患者与对照组血清BDNF及CA125水平,分析BDNF对轻型子宫内膜异位症的预测价值。结果:研究组血清BDNF及CA125水平均高于对照组,差异有统计学意义(P<0.05);轻型子宫内膜异位症患者血清BDNF及CA125水平均高于对照组,差异有统计学意义(P<0.05);与CA125水平比较,BDNF具有更大的曲线下面积(AUC),BDNF作为诊断轻型子宫内膜异位症的敏感度和特异性分别为76.2%、62.2%;重型子宫内膜异位症患者血清BDNF及CA125水平高于轻型子宫内膜异位症患者,其中BDNF水平在两组中比较差异无统计学意义(P>0.05);CA125在重度子宫内膜异位症患者中显著升高,差异有统计学意义(P<0.05)。结论:轻型子宫内膜异位症中血清BDNF升高,可作为预测轻型子宫内膜异位症的指标。

Objective To explore the expression of brain-derived neurotrophic factor(BDNF)in mild endometriosis,and to evaluate the applicability and diagnostic value of BDNF as a clinical marker of mild endometriosis.Method A total of 159 patients who were treated with laparoscopic surgery in Zhongshan Boai Hospital from 2020 to 2022 were selected as research object.They were divided into a study group(patients with endometriosis confirmed by laparoscopy and pathology,divided into mild endometriosis and severe endometriosis)of 111 cases and a control group(patients with tubal infertility)of 48 cases.A blood sample was obtained before surgery and BDNF and CA125 levels were quantified by ELISA.Then serum levels of BDNF in each group were compared,to analyze the predictive value of BDNF in mild endometriosis.Results The serum BDNF and CA125 in study group were higher than those in control group,and the difference was statistically significant(P<0.05).The serum BDNF and CA125 in patients with mild endometriosis were higher than those in control group,and the serum BDNF in patients with mild endometriosis was statistically different from that in control group(P<0.05).Compared with CA125,BDNF has larger area under the curve(AUC),The sensitivity and specificity of BDNF for the diagnosis of mild endometriosis were 76.2%and 62.2%,respectively.The serum BDNF and CA125 levels in severe endometriosis patients were higher than those in mild endometriosis patients,and there was no statistical difference in BDNF levels between the two groups(P>0.05),while CA125 levels were significantly increased in severe endometriosis patients,with statistical significance(P<0.05).Conclusion The serum BDNF of patients with mild endometriosis are elevated,which can be used to diagnose endometriosis.