长白山蚂蚁氨基酸口服液的除臭工艺优化及配方筛选

Optimization of deodorization process and formula selection for changbai mountain ant amino acid oral liquid

以蚂蚁蛋白经酶水解后得到的氨基酸酶解液为原料,对于酶解后的溶液带有异味的问题,选取β-环状糊精作为除臭剂,对酶解液进行除异味处理,得出β-环状糊精的使用量为3.0%,处理时间为2.5 h,除臭效果最佳.蚂蚁氨基酸口服液选取蔗糖、赤藓糖醇、柠檬酸三个因素进行调配,通过正交试验,确定了各因素对蚂蚁氨基酸口服液感官评分的影响顺序为A>C>B,即蔗糖添加量>柠檬酸添加量>赤藓糖醇添加量.同时,确定了口服液的最佳配方是A_(3)B_(2)C_(2),即蔗糖12%、赤藓糖醇6%、柠檬酸0.5%.本试验在单因素试验的基础上进行正交试验,再以感官评分作为指标,优化了蚂蚁氨基酸口服液的配方,最终所得到的产品酸甜可口、细腻、柔和、黄色、无沉淀.该蚂蚁氨基酸口服液,富含有17种氨基酸,游离氨基酸含量丰富,约为46.514 mg/g,其中必需氨基酸为17.135 g/L-1,占游离氨基酸总量的36.84%.此蚂蚁氨基酸口服液清香可口,方便食用,可为蚂蚁在食品方面的应用提供参考.

Used the amino acid hydrolysate obtained from the enzymatic hydrolysis of ant protein as the raw material.To solve the problem of peculiar odor in the solution after enzymatic hydrolysis,β-cyclic dextrin was selected as the deodorant,and the peculiar odor treatment was carried out on the enzymatic hydrolysate,and it was concluded that the amount ofβ-cyclic dextrin was 3.0%,the treatment time was 2.5h,and the deodorization effect was the optimal.Ant amino acid oral solution was formulated using three factors:sucrose,erythritol,and citric acid.Through orthogonal experiments,the order of influence of each factor on the sensory score of ant amino acid oral solution was determined to be A>C>B,that is,sucrose addition>citric acid addition>erythritol addition.At the same time,the optimal formula for oral liquid was determined to be A_(3) B_(2) C_(2),which includes 12%sucrose,6%erythritol,and 0.5%citric acid.On the basis of a single factor experiment,an orthogonal experiment was conducted in this experiment,and the sensory score was used as an indicator to optimize the formula of Ant Amino Acid Oral Liquid.The final product obtained was sour,sweet,delicious,delicate,soft,yellow,and sediment free.The ant amino acid oral liquid,Which is of 17 kinds of amino acids,free amino acid content,about 46.514mg/g,of which the essential amino acid is 17.135 g/L-1,accounting for 36.84%of the total free amino acids.This ant amino acid oral solution is fragrant and delicious,convenient to consume,and can provide reference for the application of ants in food.