基于转录组测序的油莎豆茎豆形成关键基因的挖掘与分析

Mining and analysis of key genes in tigernut(Cyperus esculentus L.)tubers formation based on transcriptome sequencing

【目的】通过分析油莎豆匍匐茎与新茎豆的转录组数据,挖掘与油莎豆茎豆形成相关的基因,探究油莎豆茎豆形成的分子机制。【方法】以匍匐茎及新茎豆为研究对象,通过转录组比较分析,挖掘茎豆形成相关基因,富集KEGG代谢通路。【结果】与未膨大的匍匐茎相比,膨大后的新茎豆中筛选出差异表达基因2126个,其中283个上调表达,1843个下调表达。GO富集分析显示,主要集中在胚胎发育结束于种子休眠、细胞分裂、细胞核、叶绿体、GTP酶活性等GO terms。KEGG富集分析表明,DEGs主要富集到植物激素信号转导、淀粉和蔗糖代谢通路中。其中植物激素信号转导相关基因(生长素反应因子ARF11、稻草人2-like蛋白SCR、LRR受体样丝氨酸/苏氨酸蛋白激酶At2g24230),淀粉和蔗糖代谢相关基因(葡聚糖内切-1,3-葡糖苷酶At2g27500、叶绿体异淀粉酶2 ISA2、含五肽重复序列蛋白At1g71460)在新茎豆中表达下调。上述基因可能是参与茎豆形成的相关基因。【结论】通过对新茎豆与匍匐茎进行转录组分析,挖掘出茎豆形成相关基因及代谢通路,为阐明茎豆形成的分子机制提供理论参考。

【Objective】By analysing the transcriptome data of the stolons and new tubers,to identify the genes involved in tuber formation and to explore the molecular mechanism of tigernut tuber formation.【Method】Using stolons and new tubers as the research objects,transcriptome comparative analysis was used to excavate the formation-related genes of tubers and enrich the metabolic pathway of KEGG.【Result】Compared with unexpanded stolons,2126 differentially expressed genes were screened,283 DEGs were up-regulated and 1843 DEGs were down-regulated in expanded new tubers.GO enrichment analysis showed that DEGs were mainly involved in embryo development leading to seed dormancy,cell division,nucleus,chloroplast and GTPase activity.KEGG enrichment analysis showed that the DEGs were mainly enriched in plant hormone signaling,starch and sucrose metabolism pathways.Genes related to plant hormone signaling(auxin response factor ARF11,Scarecrow 2-like protein SCR,LRR receptor serine/threonine protein kinase At2g24230)were down-regulated in new tubers.Meanwhile,genes related to starch and sucrose metabolism(glucan endo-1,3-glucosidase At2g27500,isoamylase 2,chloroplastic ISA2,pentatricopeptide repeat-containing protein At1g71460)were also down-regulated in new tubers.These genes may be involved in tuber formation.【Conclusion】Transcriptome analysis of new tubers and stolons revealed the genes involved in tuber formation and metabolic pathways,providing a theoretical reference for elucidating the molecular mechanism of tuber formation.