4个荞麦品种对盐胁迫的生理响应及抗盐性评价

Evaluation of physiological responses and salt tolerance to salt stress in four buckwheat varieties

【目的】研究不同荞麦品种对盐胁迫的生理响应及耐受性。【方法】以定甜荞1号、固荞1号、贵甜荞2号和定甜荞3号为研究对象,采用NaCl胁迫盆栽试验,以清水为对照(CK),分别对生长量(株高)、发芽率、成活率(存活率)、脯氨酸含量、可溶性糖(SS)、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、丙二醛(MDA)含量、叶绿素含量等指标进行测定,采用隶属函数法对其抗盐性进行综合分析评价。【结果】NaCl胁迫显著影响荞麦的种子萌发、幼苗生长发育和生理生化反应。4个荞麦品种抗盐性强弱依次为:贵甜荞2号>定甜荞1号>定甜荞3号>固荞1号,其中贵甜荞2号受害最轻,抗盐性最强;固荞1号受害最严重,抗盐性最弱。【结论】研究结果为盐碱地区荞麦种植的品种筛选提供一定理论依据,具有科学、全面、准确、方便的特点,有较高推广应用价值。

【Objective】To study the physiological response and tolerance of different buckwheat varieties to salt stress.【Method】Ding tian qiao No.1,Gu qiao No.1,Gui tian qiao No.2,and Ding tian qiao No.3 were used as material.Potting test by NaCl stress using clean water as a control(CK),growth amount(plant height),germination rate,survival rate,proline content,soluble sugar(SS)content,superoxide dismutase(SOD)activity,peroxidase(POD)activity,malondialdehyde(MDA)content,chlorophyll content and other indicators were determined.Principal component analysis and membership function method were used to comprehensively evaluate its salt resistance.【Result】NaCl stress significantly affected seed germination,seedling growth and development,and physiological and biochemical responses of buckwheat.The salt resistance of the four buckwheat varieties was,in order:Gui tian qiao No.2>Ding tian qiao No.1>Ding tian qiao No.3>Gu qiao No.1,among them,Gui tian qiao No.2 suffered the lightest and had the strongest salt resistance,Gu qiao No.1 is the most severely affected and the salt resistance is the weakest.【Conclusion】This paper provides some theoretical basis for the selection of buckwheat varieties to be grown in saline-alkali areas.