草坪修剪废弃物好氧和好氧-厌氧耦合堆肥中真菌菌群演替对腐殖质合成的影响

Fungal community succession in aerobic and aerobic-anaerobic coupled composting with lawn mown waste affected humus synthesis

【目的】促进草坪修剪废弃物的资源化利用和探明好氧和好氧-厌氧耦合堆肥过程中真菌群落的演替对腐殖质合成的影响。【方法】以草坪修剪废弃物为主料,设置3个处理A(草坪修剪废弃物)、B(草坪修剪废弃物+10%玉米秸秆)和C(草坪修剪废弃物+20%玉米秸秆)进行好氧和好氧-厌氧耦合堆肥。【结果】相比好氧-厌氧耦合堆肥(A),好氧堆肥(B和C)能够加速腐殖化程度,促进胡敏酸的合成,且C处理效果优于B处理。优势真菌菌群与腐殖质组分的冗余分析表明,好氧堆肥(B和C)更好地促进了木质纤维素降解真菌的相对丰度,而抑制了病原真菌的相对丰度,促进富里酸和胡敏素的降解而合成更多的胡敏酸。FUNGuild分析表明,相比好氧-厌氧耦合堆肥(A),好氧堆肥(B和C)可以灭活真菌病原体,增加腐生菌功能的相对丰度。【结论】在修剪草坪废弃物中添加10%~20%的玉米秸秆进行好氧堆肥,能够改善真菌菌群和营养模式,提高堆肥腐殖化程度,且20%的添加效果最好。

【Objective】The primary objective of the study was to promote the resource utilization of lawn mown waste and to analyze the effects of fungal community succession on humus synthesis during aerobic and aerobic-anaerobic couped composting.【Method】Lawn mown waste was used as the main material and three treatments,A(lawn mown waste alone),B(90%lawn mown waste+10%corn stover,w/w)and C(80%lawn mown waste+20%corn stover,w/w)were set up for aerobic and aerobicanaerobic coupled composting.【Result】The aerobic composting(B and C)could accelerate the humic degree and promote the synthesis of more humic acid,and C was better than B.The redundancy analysis of the dominant fungal community and humus components indicated that aerobic composting(B and C)better promoted the relative abundance of lignocellulose-degrading fungi and inhibited the relative abundance of pathogenic fungi,to promote the degradation of fulvic acid and humin to synthesize more humic acid.FUNGUILD analysis showed that aerobic composting(B and C)could effectively inactivate fungal pathogens and increase the relative abundance of saprophytic function.【Conclusion】Therefore,the addition of 10%~20%corn stover to lawn mown waste for aerobic composting could improve the flora and nutrient pattern of fungi,enhance the degree of composting humus,adding 20%corn stover to lawn mown waste was better.