具有解磷、解钾和固氮功能的甘薯茎线虫生防菌的筛选与鉴定

Screening and identification of biocontrol fungi for sweet potato stem nematodes with hydrolyze phosphorus,potassium,and nitrogen fixation functions

为筛选鉴定出具有解磷、解钾和固氮能力的甘薯茎线虫生防菌,本研究采集甘薯茎线虫发生地的甘薯根际土样,采用LB固体培养基分离出HC-6、GS3-X4等6个细菌菌株。通过初筛、复筛和性能测试,从6个菌株中筛选出一种对线虫病触杀效果好,定殖能力强,且解磷、解钾和固氮能力优秀的菌株,为GS4-S2;经生理生化特征和16S rDNA序列分析鉴定,该菌株为贝莱斯芽孢杆菌菌株,命名为HM-6。该菌株可作为防治甘薯茎线虫病的微生物菌肥研发材料,对于线虫病害防治和菌肥利用具有重要意义。

In order to screen and identify biocontrol fungi capable of phosphorus,potassium,and nitrogen fixation in sweet potato stem nematodes,soil samples from the rhizosphere of sweet potato stem nematodes in their habitat was collected.Six bacterial strains,including HC-6 and GS3-X4,were isolated using LB solid medium.Through initial screening,rescreening,and performance testing,a strain with good contact killing effect against nematode disease,strong colonization ability,and excellent phosphorus,potassium,and nitrogen fixation abilities was selected from six strains,which was GS4-S2.Based on physiological and biochemical characteristics and 16S rDNA sequence analysis,the strain was identified as Bacillus Velezensis,and named HM-6.This strain could be used as a material for the development of microbial fertilizers for the prevention and control of sweet potato stem nematode disease,which was of great significance for the prevention and control of nematode diseases and the utilization of microbial fertilizers.