乳没软膏治疗急性闭合性软组织损伤大鼠的抗炎研究

Anti-inflammatory Effect of Rumo Ointment in the Treatment of Acute Closed Soft Tissue Injury of Rats

目的:研究乳没软膏治疗急性闭合性软组织损伤的抗炎镇痛作用。方法:60只斯泼累格·多雷(SD)大鼠(6只备用),分为A组(空白对照组)、B组(模型对照组)、C组(青鹏软膏组)、D组(乳没软膏高剂量组)、E组(乳没软膏中剂量组)、F组(乳没软膏低剂量组);通过建立SD大鼠急性闭合性软组织损伤模型,检测受损组织内肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、前列腺素2(PGE-2)、超氧化物歧化酶(SOD)含量的变化;镜下观察受损组织形态学变化及组织中碱性细胞生长因子(bFGF)含量变化。结果:造模药物干预后,造模组大鼠损伤组织TNF-α、IL-1β、PGE-2含量逐渐下降,其中以C组、D组下降最为明显,B组下降最为缓慢;SOD含量以C组、D组的上升趋势最为明显,B组上升最为缓慢。造模后第2天,各组bFGF蛋白平均光密度值总体比较差异无统计学意义(P>0.05);造模后第7天、第14天,各组bFGF蛋白平均光密度值总体比较差异有统计学意义(P<0.05)。结论:乳没软膏高剂量组与青鹏软膏在抗炎镇痛方面效果相当。

Objective:To study the anti-inflammatory and analgesic effects of Rumo ointment in the treatment of acute closed soft tissue injury.Methods:Sixty SD rats(6 spare)were divided into group A(blank control group),group B(model control group),group C(Qingpeng ointment group),group D(high-dose Rumo ointment group),group E(middle-dose Rumo ointment group),and group F(low-dose Rumo ointment group).By establishing the SD rat model of acute closed soft tissue injury,the content of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),prostaglandin E 2(PGE 2),and superoxide dismutase(SOD)was detected in the damaged tissue.The morphological changes of the damaged tissue and the content changes of basic fibroblast growth factor(bFGF)in the tissue were observed under a microscope.Results:After intervention using modeling drugs,the content of TNF-α,IL-1β,and PGE-2 in the injured tissues of rats in the modeling groups gradually decreased,among which the decrease in group C and group D was the most obvious,and the decrease in group B was the slowest.The increase in SOD content was the most obvious in group C and group D,and that in group B was the slowest.On the second day after modeling,there was no significant difference in the average optical density of bFGF protein in each group(P>0.05).On the 7th and 14th days after modeling,the average optical density of bFGF protein in each group was statistically different(P<0.05).Conclusion:High-dose Rumo ointment and Qingpeng ointment have comparable anti-inflammatory and analgesic effects.