摘要
It is still a huge challenge for bone regenerative biomaterial to balance its mechanical,biological and biodegradable properties.In the present study,a new composite material including treated dentin matrix(TDM)andα-calcium sulphate hemihydrate(α-CSH)was prepared.The optimal composition ratio between TDM andα-CSH was explored.The results indicate that both components were physically mixed and structurally stable.Its compressive strength reaches up to 5.027±0.035 MPa for 50%TDM/α-CSH group,similar to human cancellous bone tissues.Biological experiments results show that TDM/α-CSH composite exhibits excellent biocompatibility and the expression of osteogenic related genes and proteins(ALP,RUNX2,OPN)is significantly increased.In vivo experiments suggest that the addition of TDM for each group(10%,30%,50%)effectively promotes cell proliferation and osteomalacia.In addition,50%of the TDM/α-CSH combination displays optimal osteoconductivity.The novel TDM/α-CSH composite is a good candidate for certain applications in bone tissue engineering.
基金
the National Natural Science Foundation of China(grant numbers 31670994,U1904145 and 81901039)
Nature Science Foundation of Henan Province(grant numbers 182300410340,222300420569)
Henan Medical Science and Technology Research Project(grant number SBGJ202002073,SBGJ202002075)
Science and Technology Department of Science and Technology Research(grant number 182102310456)
Henan Province Middle-Aged And Young Health Science and Technology Innovation Leader Training Project(grant number YXKC2021014)
Scientific Research and Innovation Team of The First Affiliated Hospital of Zhengzhou University(grant number QNCXTD2023021)
Union project of Medical and Technology Research Program of Henan Province(grant number LHGJ20190191).
