慢性宫颈炎合并HPV感染140例血清SOX2和PD-L1表达与临床病理特征相关性分析

Correlation between serum SOX2 and PD-L1 expression and clinicopathological characteristics in 140 patients with chronic cervicitis complicated with HPV infection

目的探究慢性宫颈炎合并人乳头状瘤病毒(HPV)感染病人血清性别决定区Y框蛋白2(SOX2)和程序性死亡配体1(PD-L1)表达与临床病理特征相关性。方法选取2020年10月至2022年10月在重庆市江津区中医院就诊治疗的未合并HPV感染慢性宫颈炎病人160例作为慢性宫颈炎组,慢性宫颈炎合并HPV感染病人140例作为合并HPV组,另选取在该院体检健康女性110例作为对照组。采用酶联免疫吸附测定(ELISA)检测研究对象血清SOX2水平,实时荧光定量PCR(qRT-PCR)测定血清PD-L1 mRNA水平,二代杂交捕获实验技术(HC2)检测HPV-DNA。结果对照组、慢性宫颈炎组、合并HPV组血清SOX2、PD-L1mRNA水平呈现逐渐升高的趋势(P<0.05);低危型HPV组、中危型HPV组、高危型HPV组血清SOX2、PD-L1 mRNA水平呈现逐渐升高的趋势(P<0.05);慢性宫颈炎合并HPV病人中单一感染率为45.71%(64/140),多重感染率为54.29%(76/140)(P>0.05),其中慢性宫颈炎合并HPV病人中低危型单一感染与多重感染病例数差异有统计学意义(P<0.05);高水平SOX2与低水平SOX2以及高水平PD-L1 mRNA与低水平PD-L1 mRNA慢性宫颈炎病人合并HPV率差异有统计学意义(P<0.05)。结论慢性宫颈炎合并HPV感染病人血清SOX2、PD-L1 mRNA水平均显著升高,与HPV分型密切相关,且病人血清SOX2、PD-L1 mRNA水平与HPV感染均呈正相关。

Objective To investigate the correlation between the expression of serum sex-determining region of Y chromosome-related high-mobility-group box 2(SOX2),programmed death ligand-1(PD-L1)in patients with chronic cervicitis complicated with human papillomavirus(HPV)infection and the clinicopathological characteristics.Methods A total of 160 cases of chronic cervicitis without HPV infection treated in Jiangjin District Hospital of Traditional Chinese Medicine from October 2020 to October 2022 were selected as the chronic cervicitis group,and 140 cases of chronic cervicitis with HPV infection were selected as the HPV group,another 110 healthy women who underwent physical checkup in our hospital were selected as the control group.The serum SOX2 level of the subjects was measured by enzyme-linked immunosorbent assay(ELISA),real-time fluorescence quantitative PCR(qRT-PCR)was applied to detect the level of PD-L1 mRNA in serum,and the HPV-DNA was detected by second-generation hybridization capture assay(HC2).Results The levels of serum SOX2 and PD-L1 mRNA in control group,chronic cervicitis group and HPV group showed a gradually increasing trend(P<0.05).The levels of serum SOX2 and PD-L1 mRNA in low-risk HPV group,medium-risk HPV group and high-risk HPV group showed a gradually increasing trend(P<0.05).The single infection rate in patients with chronic cervicitis complicated with HPV was 45.71%(64/140),and the multiple infection rate was 54.29%(76/140)(P>0.05).There was a statistically significant difference in the numbers of single infection and multiple infection in patients with chronic cervicitis complicated with HPV(P<0.05).There were significant differences in the rate of HPV in patients with chronic cervicitis between high SOX2 level and low SOX2 level,and between high PD-L1 mRNA level and low PD-L1 mRNA level.Conclusion The levels of serum SOX2 and PD-L1 mRNA in patients with chronic cervicitis complicated by HPV infection are obviously increased,which is closely related to HPV typing,and the levels of serum SOX2 and PD-L1 mRNA are positively correlated with HPV infection.