p-AKT与VEGF、HER-2在膀胱浸润性尿路上皮癌中的表达及其与患者预后关系研究

Correlation between p-AKT,VEGF,HER-2 expression and prognosis of bladder invasive urothelial carcinoma

目的探讨磷酸化蛋白激酶B(p-AKT)与血管内皮生长因子(VEGF)、人表皮生长因子受体2(HER-2)在膀胱浸润性尿路上皮癌中的表达及其与患者预后的关系。方法用免疫组织化学染色(IHC)和荧光原位杂交(FISH)检测69例膀胱浸润性尿路上皮癌、20例膀胱黏膜良性组织(慢性炎症、乳头状瘤组织)中p-AKT、VEGF、HER-2蛋白的表达。结果膀胱浸润性尿路上皮癌中p-AKT、VEGF、HER-2表达均显著高于膀胱良性组织的表达(P<0.05);p-AKT、VEGF和HER-2在高级别浸润性尿路上皮癌中的表达均显著高于低级别浸润性尿路上皮癌中的表达(P<0.05);p-AKT在后期复发的患者中的表达高于未复发患者(P<0.05);浸润性尿路上皮癌患者中p-AKT与VEGF、HER-2的表达均呈显著正相关(P<0.05);p-AKT、VEGF和HER-2蛋白表达阳性的患者生存率均明显低于阴性表达患者的生存率(P<0.05)。结论p-AKT、VEGF及HER-2蛋白均可能参与了膀胱浸润性尿路上皮癌的发生发展,并且与患者的预后相关,可能成为膀胱尿路上皮癌的预后指标;膀胱浸润性尿路上皮癌中p-AKT与VEGF、HER-2可能存在相互调节关系。

Objective To investigate the expression of phosphorylated protein kinase B(p-AKT),vascular endothelial growth factor(VEGF)and human epidermal growth factor receptor 2(HER-2)in bladder invasive urothelial carcinoma and its relationship with the prognosis.Methods Immunohistochemistry staining(IHC)and fluorescence in situ hybridization(FISH)were used to detect the expression of p-AKT,VEGF and HER-2 proteins in 69 cases of bladder invasive urothelial carcinoma and 20 cases of benign bladder mucosa(chronic inflammation,papilloma tissue).Results The expression of p-AKT,VEGF and HER-2 in bladder invasive urothelial carcinoma was significantly higher than those in benign bladder tissues(P<0.05).The expression of p-AKT,VEGF and HER-2 in high-grade invasive urothelial carcinoma was significantly higher than those in low-grade invasive urothelial carcinoma(P<0.05).The expression of p-AKT in patients with later recurrence was higher than that without recurrence(P<0.05).The expression of p-AKT was positively correlated with the expression of VEGF and HER-2 in invasive urothelial carcinoma(P<0.05).The survival rate of patients with positive expression of p-AKT,VEGF and HER-2 protein was significantly lower than negative expression(P<0.05).Conclusions p-AKT,VEGF and HER-2 proteins may be involved in the occurrence and development of bladder invasive urothelial carcinoma,and are related to the prognosis of patients,and may become prognostic indicators of bladder urothelial carcinoma.There may be a mutual regulatory relationship between p-AKT,VEGF and HER-2 in bladder invasive urothelial carcinoma.