miR-299-5p联合CD44对儿童非综合征型唇腭裂的诊断价值

Diagnostic Value of miR-299-5p Combined with CD44 in Children with Nonsyndromic Cleft Lip and Palate

目的探究miR-299-5p联合CD44对儿童非综合征型唇腭裂的诊断价值。方法选择濮阳市第三人民医院2019年7月至2022年7月收治的非综合征型唇腭裂患儿80例,其中唇裂伴或不伴腭裂患儿40例记为A组,单纯腭裂患儿40例记为B组。另选取医院同期体检的健康患儿40例记为C组。分别采用实时荧光定量RT-PCR法和免疫印迹法测定受试者的miR-299-5p、CD44的相对表达量。绘制受试者工作特征(ROC)曲线,并根据曲线下面积(AUC)评估miR-299-5p、CD44对儿童非综合征型唇腭裂的诊断效能。结果A、B组的miR-299-5p相对表达量均低于C组,差异有统计学意义(P<0.05)。A、B组的CD44相对表达量均低于C组,差异有统计学意义(P<0.05)。ROC分析结果显示,miR-299-5p、CD44单一及联合诊断儿童非综合征型唇腭裂的灵敏度分别为72.50%、62.50%、82.50%;特异度分别为72.50%、82.50%、80.00%;AUC分别为0.716(95%CI:0.573~0.859)、0.718(95%CI:0.572~0.862)、0.821(95%CI:0.716~0.965),且二者联合预测效能高于各指标单一预测效能(P<0.05)。结论miR-299-5p、CD44在儿童非综合征型唇腭裂中的诊断效能良好。

Objective To explore the diagnostic value of miR-299-5p combined with CD44 in children with nonsyndromic cleft lip and palate.Methods Eighty children with non syndromic cleft lip and palate admitted to the Third People’s Hospital of Puyang from July 2019 to July 2022 were selected.Among them,40 children with cleft lip or without cleft palate were recorded as group A,and 40 children with simple cleft palate were recorded as group B.Another 40 healthy children during the same period of physical examination in hospital were recorded as group C.The relative expression levels of miR-299-5p and CD44 were measured by real-time fluorescence quantitative RT-PCR and western blot,respectively.The receiver operating characteristic(ROC)of the subjects was drawn,and the diagnostic efficacy of miR-299-5p and CD44 in children with nonsyndromic cleft lip and palate was evaluated according to the area under the curve(AUC).Results The relative expression of miR-299-5p in group A and group B was lower than that in group C,and the difference was statistically significant(P<0.05).The relative expression of CD44 in group A and group B was lower than that in group C,the differences were statistically significant(P<0.05).ROC analysis showed that the sensitivity of miR-299-5p and CD44 in the diagnosis of nonsyndromic cleft lip and palate in children were 72.50%,62.50%and 82.50%.The specificity were 72.50%,82.50%and 80.00%respectively.AUC were 0.716(95%CI:0.573-0.859),0.718(95%CI:0.572-0.862),0.821(95%CI:0.716-0.965),respectively,and their combined prediction efficiency was higher than that of single index(P<0.05).Conclusion The diagnostic efficacy of miR-299-5p and CD44 in children with non syndromic cleft lip and palate is good.