亚甲基蓝测定不同效价肝素钠含量的研究

Study on the Determination of Heparin Sodium Contents of Different Potencies by Methylene Blue

目的:对不同效价肝素钠的含量和活性进行评价。方法:建立了测定肝素钠浓度的紫外分光光度法,并探究了不同效价肝素钠对测定的影响。结果:在664 nm波长下,当肝素钠浓度在0~2.00 mg·L^(-1)范围内,其与等量亚甲基蓝反应后,所测得的吸光度与肝素钠的浓度呈良好的线性关系,150 USP units·mg^(-1)、185 USP units·mg^(-1)和205 USP units·mg^(-1)肝素钠线性方程的相关系数分别为0.9996、0.9992和0.9990。研究发现肝素钠的效价与线性方程的斜率存在正相关关系,即随着肝素钠的效价的增加,斜率亦呈增加趋势。结论:本研究建立的方法可以对大批量样品进行简便、快速、易于控制的肝素钠定量测定并能间接探究肝素的活性。

Objective:Evaluation of the content and activity of heparin sodium with different potencies.Methods:An ultraviolet spectrophotometric method for the determination of heparin sodium concentration was developed and the effect of different potencies of heparin sodium on the determination was investigated.Results:At the wavelength of 664 nm,the absorbance measured after its reaction with an equal amount of methylene blue showed a good linear relationship with the concentration of sodium heparin when the concentration of heparin sodium was in the range of 0-2.00 mg·L^(-1).The correlation coefficients of the linear equations for 150 USP units·mg^(-1),185 USP units·mg^(-1),and 205 USP units·mg^(-1)heparin sodium were 0.9996,0.9992,and 0.9990,respectively.In addition,it was found that there was a positive correlation between the potency of heparin sodium and the slope of the linear equation,that is,as the potency of heparin sodium increased,the slope also increased.Conclusion:The method developed in this study allows for simple,rapid,and easily controlled quantitative determination of heparin and indirect investigation of heparin activity in large samples.