新疆马铃薯黑痣病病原菌的分离及鉴定

Isolation and identification of the pathogen for potato black scurf in Xinjiang

【目的】分离鉴定引起新疆马铃薯黑痣病病原菌,为筛选防治该病药剂奠定基础。【方法】从新疆吉木萨尔县、奇台县、乌鲁木齐市达坂城区和昭苏县等马铃薯种植区采集马铃薯块茎,通过组织分离法从带有马铃薯黑痣病的块茎分离病原菌,利用电子显微镜观察病原菌的形态特征,进行对峙培养和ITS序列分析,鉴定融合群。【结果】从新疆吉木萨尔县、奇台县、乌鲁木齐市达坂城区和昭苏县分离的4株病原菌均能使马铃薯块茎出现马铃薯黑痣病症状,分离菌株均为AG3融合群。【结论】AG3融合群是新疆马铃薯黑痣病的主要致病群。

【Objective】To isolate and identify the pathogen causing potato black scurf in Xinjiang in the hope of providing a foundation for screening of control agents and establishment of control technology.【Methods】Potato tubers were collected from Jimsar,Qitai,Dabancheng and Zhaosu in Xinjiang,and Rhizoctonia solani were isolated from tubers with potato black scurf by tissue separation method.Anastomosis groups of Rhizoctonia solani were identified by paired culture and ITS sequence analysis.【Results】The four strains isolated from Jimsar,Qitai,Dabancheng and Zhaosu were all able to cause the symptoms of potato black scurf on potato tubers.The isolated strains were Anastomosis groups AG3.【Conclusion】Anastomosis Groups AG3 is the main pathogenic group of potato black scurf in Xinjiang.