摘要
Infectious diseases caused by pathogenic bacteria are a serious threat to global public health.Nowadays,antibiotics and other clinical drugs suffer from some fundamental disadvantages such as narrow-spectrum antibacterial effect and the risk to induce drug resistance,while inorganic functional nanomaterials with biological catalytic activities have been developed as novel antibacterial agents.In this study,we prepared two-dimensional ultrathin vanadium oxide nanosheets(VO_(x)NSs)with mixed valence states from the oxidised layer of vanadium powder(bulk V)by ultrasonically assisted liquid exfoliation.By conveniently switching between V^(IV)and V^(V),VO_(x)NSs can efficiently catalyse H_(2)O_(2)enriched in bacterially infected areas to generate hydroxyl radicals(·OH),which induce bacterial oxidative stress and apoptosis.This process can occur in both weakly alkaline and acidic environments,thus being independent of the pH value in infection areas.In addition,contributed by the intrinsic characteristics of vanadium,two-dimensional morphology and reasonable valence state ratios,VO_(x)NSs exhibit the advantages of broad antibacterial spectrum,high catalytic activity and non-toxic by-products.This novel nanosheet offers a new strategy to heal infected wounds and extends the application of nanocatalytic medicine towards anti-infection.
致病菌引起的感染疾病是对全球公共卫生的严重威胁.目前,抗生素等临床药物存在抗菌作用窄谱、易诱发耐药等根本缺点,而具有生物催化活性的无机纳米功能材料已被用作新型抗菌剂.本研究利用超声辅助液相剥离法,以钒粉(bulk V)的氧化层为原料制备了混合价态二维超薄氧化钒纳米片(VO_(x)NSs),其通过正四和正五价钒的相互转换,可高效催化感染区域富集的H_(2)O_(2)生成羟基自由基(hydroxyl radicals,·OH),诱导细菌氧化应激和凋亡.此过程在弱碱和弱酸性环境均可发生,因此不受感染区域pH制约.此外,由于钒的固有特性、二维形貌和合理的价态比,VO_(x)NSs具有广谱抗菌、催化活性高和副产物无毒等优点.这种新型纳米片提供了治疗感染伤口的新策略,扩展了纳米催化医学在抗感染方面的应用.
作者
Zhimin Zhang
Zhao Guo
Zesong Ruan
Min Ge
Shibo Cao
Jiayi Yuan
Zhen Xu
Lieying Fan
Ming Zong
Han Lin
Jianlin Shi
张志民;郭钊;阮泽松;葛敏;曹士博;袁佳仪;徐甄;范列英;宗明;林翰;施剑林(State Key Laboratory of High Performance Ceramics and Superfine Microstructures,Shanghai Institute of Ceramics Chinese Academy of Sciences,Research Unit of Nanocatalytic Medicine in Specific Therapy for Serious Disease,Chinese Academy of Medical Sciences,Shanghai,200050,China;Department of Orthopedic Surgery,Shanghai Institute of Microsurgery on Extremities,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai,200233,China;Department of Clinical Laboratory,Shanghai East Hospital,Tongji University School of Medicine,Shanghai,200120,China;Department of Electrical and Electronic Engineering,The University of Hong Kong,Hong Kong,China;Shanghai Tenth People’s Hospital,Shanghai Frontiers Science Center of Nanocatalytic Medicine,School of Medicine,Tongji University,Shanghai,200331,China;Center of Materials Science and Optoelectronics Engineering,University of Chinese Academy of Sciences,Beijing,100049,China)
基金
financial support from the National Key R&D Program of China(Grant No.2022YFB3804500)
Shanghai Pilot Program for Basic Research-Chinese Academy of Science,Shanghai Branch(Grant No.JCYJ-SHFY-2022-003)
National Natural Science Foundation of China(Grant No.52372276,52002391 and 22335006)
Basic Research Program of Shanghai Municipal Government(Grant No.21JC1406000)
Youth Innovation Promotion Association CAS(Grant No.2023262)
Young Elite Scientists Sponsorship Program by CAST(Grant No.YESS20210149)
Shanghai Science and Technology Committee Rising-Star Program(Grant No.22QA1410200)
Academic Leaders Training Program of Pudong Health Bureau of Shanghai(Grant No.PWRd2022-03),Academic Leaders Training Program of Pudong Health Bureau of Shanghai(Grant No.2022-3)
shanghai Science and Technology Innovation Action Plan(Grant No.21Y11900800)
Shanghai Municipal Health and Planning Commission(No.202150010)。
