外源NO对香菇热胁迫的缓解机制研究

Alleviation mechanism of exogenous nitric oxide on heat stress of Lentinus edodes

为探究外源NO对香菇热胁迫的缓解机制,以香菇L135菌株为试验材料,研究外源添加NO对香菇菌丝高温胁迫下氧化损伤的缓解效应。结果表明,高温胁迫明显抑制了香菇菌丝的生长,导致菌丝蜷曲甚至断裂,添加适宜浓度(800μmol·L^(-1))的NO供体(SNP)处理可以有效缓解热胁迫条件下的形态伤害;外源添加SNP处理组与未添加处理组相比,菌丝细胞内过氧化氢及丙二醛的含量均显著下降,热激48和72 h分别下降了17.48%、35.24%和16.70%、19.87%,且胞内超氧化物歧化酶、过氧化物酶和过氧化氢酶活性均显著提高。综上,添加800μmol·L-1的外源NO能够有效缓解香菇热胁迫,为进一步阐明食用菌抗高温胁迫机制奠定了理论基础。

To investigate the mechanism of exogenous NO alleviating heat stress in Lentinula edodes,L.edodes strain L135 was used as the experimental material to study the alleviating effect of exogenous NO on oxidative damage of L.edodes hyphae under high temperature stress.The results show that high temperature stress significantly inhibited the growth of L.edodes mycelia,caused mycelia curl or even break,SNPtreatment with appropriate concentration(800μmol·L^(-1))could effectively alleviate the morphological damage caused by heat stress;the levels of H2O2 and MDA content in mycelial cells decreased significantly in the group treated with exogenous SNP compared with the group treated without SNP,heating shock 48 and 72 h decreased by 17.48%,35.24%and 16.70%,19.87%respectively,and the intracellular activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activity were significantly increased.In conclusion,the addition of 800μmol·L-1 exogenous NO can effectively alleviate heat stress in L.edodes,laying a theoretical foundation for further elucidating the mechanism of high-temperature stress resistance in edible fungi.