百香果SPL转录因子家族成员鉴定及其对低温胁迫的响应

Identification of SPL transcription factor gene family members and their response to cold stress in passion fruit

SPL(SQUAMOSA启动子结合类蛋白质)是一类特异存在于植物中的转录因子,参与植物生长发育的多个过程。为明确百香果SPL转录因子家族成员特征及其对低温胁迫的响应,本研究利用生物信息学方法和百香果基因组数据,对百香果SPL家族基因的结构、染色体分布、共线性关系及其编码蛋白质理化性质、进化关系进行分析,并通过低温和常温处理明确了百香果幼苗叶片SPL家族基因的相对表达量差异。结果表明,从百香果基因组中鉴定出19个SPL家族成员,分布于7条染色体上,其中包含3对共线性基因对。系统进化分析结果显示该家族分为9个亚族。百香果PeSPL基因启动子区域存在大量激素响应元件和逆境胁迫响应元件。低温胁迫下,百香果PeSPL2、PeSPL3、PeSPL6、PeSPL7、PeSPL8和PeSPL96个基因的相对表达量极显著上升,PeSPL13和PeSPL182个基因的相对表达量显著下降。本研究结果可为进一步揭示百香果SPL基因对低温胁迫的响应机制提供依据。

SPL(SQUAMOSA promoter-binding protein-like)is a plant-specific transcription factor that participates in many processes of plant growth and development.In order to clarify the characteristics of SPL transcription factor family members and their response to low temperature stress in passion fruit,bioinformatics methods and genome data of passion fruit were used to analyze the structure,chromosome distribution,collinear relationship of SPL family genes in passion fruit,as well as the physicochemical properties and evolutionary relationship of SPL family genes encoded proteins.The relative expression differences of SPL family genes in the leaves of passion fruit seedlings were clarified by low temperature treatment and normal temperature treatment.The results showed that 19 SPL family members were identified from the genome of passion fruit,which were distributed on seven chromosomes,including three pairs of collinear gene pairs.SPL gene family of passion fruit was divided into nine branches according to results of phylogenetic tree.There were many cis-acting elements in the promoter region of PeSPL genes of passion fruit,which responsed to hormones and stress.Under low temperature stress,the relative expression levels of PeSPL2,PeSPL3,PeSPL6,PeSPL7,PeSPL8 and PeSPL9 were extremely significantly increased,and the relative expression levels of PeSPL13 and PeSPL18 were significantly decreased.The results of this study can provide a basis for further revealing the response mechanism of passion fruit SPL gene to low temperature stress.