超声诱发成核提高HepG2细胞冷冻消融致死率研究

Improving the lethality of cryoablation in HepG2 cells by usingultrasound-induced nucleation

冷冻消融技术对于治疗实体肿瘤具有一定的效果,但普遍存在降温效果有限、靶向杀伤效率不高等问题。为提高冷冻消融的疗效,提出了将超声诱发成核用于冷冻消融的一种新的辅助手段,研究了2种降温速率下不同诱发成核温度对细胞存活率的影响,观察并记录了超声诱发成核后的胞外冰晶形态、胞内冰晶生成情况及复温后的细胞损伤情况。结果表明:使用超声诱发成核辅助冷冻消融可以提高肿瘤细胞的致死率。慢速冷冻辅助成核组的效果最佳,消融更为彻底,HepG2细胞的存活率低至15.60%±2.60%。快速冷冻辅助成核时,需要配合较低的诱发成核温度才能更好地杀伤细胞。设计开发具有超声诱发成核功能的冷冻消融器械具有实际意义。

Cryoablation techniques are effective for the treatment of solid tumors,but generally suffer from the limited cooling effect and low target killing efficiency.To improve the efficacy,the use of ultrasound-induced nucleation was proposed as a new adjunct.The effects of different induced nucleation temperatures on the cell viability at two cooling rates were investigated,and the morphology of extracellular ice crystals,intracellular ice crystal generation and cell damage after rewarming were observed and recorded.The results show that the use of ultrasound-induced nucleation as an adjunct to cryoablation can improve the lethality of tumor cells.The slow freezing group with ultrasound-induced nucleation have the best results with more complete ablation and lower cell viability of 15.60%±2.60%.The rapid freezing group need to be coupled with a lower induced nucleation temperature to better kill the cells.The design and development of cryoablation devices with ultrasound-induced nucleation have practical implications.