抗抑霉唑葡萄白腐病菌的适合度及与三唑类杀菌剂的交互抗性研究

Study on Fitness of Imazalil-resistant Coniella vitis and Its Cross-resistance with Triazole Fungicides

以对抑霉唑不同敏感性的葡萄白腐病菌菌株为试材,研究抗抑霉唑菌株的适合度及抑霉唑与常用三唑类杀菌剂的交互抗性。结果显示,在菌丝生长速率、产孢量、致病力方面,部分抗性菌株显著高于敏感菌株,但致病力与菌株的EC_(50)值无显著相关性。37个葡萄白腐病菌菌株对抑霉唑、丙环唑、苯醚甲环唑、氟硅唑的平均EC_(50)值分别为16.5339、1.1159、2.2293、1.6481μg·mL^(-1),抑霉唑与丙环唑、苯醚甲环唑、氟硅唑的EC_(50)对数值之间的相关系数分别为0.6572、0.5261、0.5106,表明抑霉唑与丙环唑存在正交互抗性,与苯醚甲环唑、氟硅唑无显著交互抗性。本研究表明,对抑霉唑产生抗性的葡萄白腐病菌菌株具有较强的适合度,存在形成优势群体的潜在性,因此,用抑霉唑防治葡萄白腐病时,应尽量避免与丙环唑混合使用,以延缓其抗药性的发展。

The fitness of Coniella vitis resistant to imazalil and cross-resistance between imazalil and triazole fungicides were studied.The results showed that some resistant strains were significantly higher than sensitive strains in terms of mycelial growth rate,spore production and pathogenicity,but there was no significant correlation between pathogenicity and the EC_(50)value of strain.The average EC_(50)value of 37 strains of Coniella vitis to imazalil,propiconazole,difenoconazole and flusilazole were 16.5339,1.1159,2.2293,1.6481μg·mL^(-1),respectively.The correlation coefficients of EC_(50)logarithm of imazalil with propiconazole,difenoconazole and flusilazole were 0.6572,0.5261,and 0.5106 respectively,indicating that there was cross-resistance between imazalil and propiconazole,but no significant cross-resistance with difenoconazole and flusilazole.These results showed that the Coniella vitis resistant to imazalil had strong fitness,and had the potential to become a dominant population.Therefore,when using imazalil to control grape white rot,it is important to avoid mixing it with propiconazole,in order to slow down the development of pathogen resistance.