凝胶色谱法进行聚乙烯吡咯烷酮的含量测定

Determination of Polyvinylpyrrolidone by Gel Chromatography

建立了凝胶色谱-紫外法测定聚乙烯吡咯烷酮含量的方法,并经方法学验证其具有良好的专属可行性。以0.1 mol/L氯化钠/乙腈(7∶3,V∶V)作为流动相,采用聚合物基质凝胶色谱柱,检测波长205 nm。聚乙烯吡咯烷酮的质量浓度在0.1~1.5 mg/mL范围内与色谱峰面积呈良好的线性关系,线性相关系数为0.9999。聚乙烯吡咯烷酮的高、中、低3种质量浓度的回收率为99.3%~102.0%,相对标准偏差(RSD)为0.35%~0.81%,符合测定要求,准确度良好。

A gel chromatography-ultraviolet method for determining polyvinylpyrrolidone content was established,and it was proved to possess good exclusive feasibility.The detection wavelength is 205 nm on a polymer gel column with 0.1 mol/L sodium chloride as mobile phase and acetonitrile(7∶3,V∶V)as mobile phase.The mass concentration of polyvinylpyrrolidone shows a good linear relationship with peak area in the range of 0.1~1.5 mg/mL,and the linear correlation coefficient is0.9999.The recoveries are 99.3%~102.0%and relative standard deviation(RSD)is 0.35%~0.81%at high,medium and low concentrations,which can meet the determination requirements with good accuracy.This established gel chromatography method is accurate,simple and suitable for the determination of polyvinylpyrrolidone in different applications.